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发光二极管的光谱校正允许使用窄带扩散反射光谱学准确计算水化比率

Jesse H Lam¹, Jeonghun Kim^2,3, Kelsey J Tu²

¹Dankook University, Beckman Laser Institute Korea, School of Medicine, Cheonan, Republic of Korea.

Journal of biomedical optics

|August 2, 2023

概括

光谱校正 (SC) 算法通过考虑LED宽带宽来改善基于LED的组织光谱学. 这种方法提高了染色体度计算的准确性,特别是在900-1000纳米范围内.

科学领域:

生物医学光学生物医学光学
频谱学是一种光谱学.
医疗成像医学成像

背景情况:

发光二极管 (LED) 对组织光谱学具有成本效益,但具有广泛的光谱带宽.
这种宽度使精确的染色体度计算变得复杂,特别是在900-1000nm窗口中,由于光谱交叉声.
现有的窄带扩散反射光谱 (nb-DRS) 通常需要复杂的宽带光源和光谱仪.

研究的目的:

引入和验证用于基于LED的组织光谱的光谱校正 (SC) 算法.
为了证明SC在减轻LED光谱带宽引起的误差方面的有效性.
为了实现一个简化的nb-DRS系统,只使用LED和光二极管探测器.

主要方法:

开发并应用了一种光谱校正 (SC) 算法来计算LED光谱带宽.
使用窄带扩散反射光谱 (nb-DRS) 采用简化的硬件设置 (五个LED和一个光电极管).
通过模拟,乳液幻影和人体组织分析验证了SC方法.

主要成果:

模拟证实SC可以纠正来自LED光谱带宽的错误.
在乳液幻影中,SC将平均和最大误差从8.7% (14%) 降低到2.2% (6.4%).
该SC算法使一个简化的nb-DRS系统能够实现与较大的宽带系统可比的性能.

结论:

光谱校正 (SC) 有效地弥补了组织光谱学中LED的光谱带宽.
这种方法允许使用简化,低成本的基于LED的nb-DRS系统进行准确的染色体度分析.
经过验证的SC算法增强了LED在生物组织中的定量光学光谱学的实用性.

关键词:

补充水分补充水分补充水分发光二极管的光谱校正狭带扩散反射光谱学狭带扩散反射光谱学

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