通过预mRNA处理机器介导的Vv-circSIZ1有助于盐分耐受性
Zhen Gao1, Baozhen Sun1, Zongbao Fan1
1State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, Shandong, 271018, China.
The New phytologist
|August 2, 2023
概括
这项研究揭示了一种新的葡萄圆形RNA,Vv-circSIZ1,对植物的盐分耐受性至关重要. 它的生物发生和功能得到了阐明,突出了它在改善植物抗压能力方面的作用.
科学领域:
- 植物分子生物学 植物分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 生物化学 生物化学
背景情况:
- 循环RNAs (circRNAs) 在植物中普遍存在,但它们的生物发生和功能作用尚不清楚.
- 了解circRNA调节是推动植物科学和作物改进的关键.
研究的目的:
- 为了研究一种新的葡萄circRNA,Vv-circSIZ1.Z的生物发生和抗盐功能.
- 阐明管理Vv-circSIZ1表达和活动的调控机制.
主要方法:
- 表达等离子体和遗传转变的广泛突变发生.
- 分析不同植物物种 (葡萄,尼科蒂亚娜·本塔米亚娜*,阿拉比多普西斯*) 中的circRNA表达.
- 在circRNA生物发生过程中研究侧边内子,拼接信号,拼接酶体活性和RNA结合蛋白的作用.
主要成果:
- 识别了Vv-circSIZ1,主要表达在葡萄树叶细胞质中,具有特定物种的特征.
- 证明侧边内子保留长度和Vv-circSIZ1生成效率之间存在正相关性.
- 证明了结合体活性与Vv-circSIZ1表达相反,并且RNA结合蛋白调节其表达.
- 过度表达Vv-circSIZ1增加了葡萄和N. benthamiana的盐耐受性.
- Vv-circSIZ1减轻了VvmiR3631介导的VvVHAc1抑制,并促进了父母基因转录.
结论:
- 阐明了Vv-circSIZ1的生物发生机制,强调侧翼内子和拼接信号的作用.
- 确立了Vv-circSIZ1作为一种功能性的circRNA,它赋予了盐分耐受性.
- 揭示了Vv-circSIZ1在miRNA路径和基因转录中的调节作用,扩大了对植物circRNA的理解.
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