鉴定塔利山羊品种某些热冲击蛋白基因的遗传变异,并研究它们对相关蛋白质的结构和功能影响
Elham Rezvannejad1, SeyedAzim Mousavizadeh2
1Department of Biotechnology, Institute of Sciences and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
Veterinary medicine and science
|August 2, 2023
概括
热冲击蛋白 (HSP) 的遗传变异影响塔利山羊适应热量. 在HSP基因中的特定nsSNP,如HSP70A4中的rs669769139,与蛋白质功能改变和气候适应性有关.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 动物遗传学动物遗传学
背景情况:
- 动物通过遗传和表型变化适应环境挑战.
- 热冲击蛋白 (HSP) 在细胞应激反应和适应中起着至关重要的作用.
研究的目的:
- 研究10个HSP基因中的遗传变异对塔利山羊蛋白质结构和功能的影响.
- 为了将这些变异与热敏的桑恩山羊品种的变异进行比较.
主要方法:
- 在塔利山羊中对10个HSP基因进行DNA测序和生物信息学分析.
- 使用多种在线工具识别和描述非同义单核酸多态 (nsSNP).
- 将nsSNP与Saanen山羊进行比较,以评估功能影响.
主要成果:
- 在塔利山羊的HSP基因中确定了三种有害的和七种自然错误的nsSNP.
- 在HSP70A4,HSP70A9,HSP40C17,HSP40C27和HSPD1中检测到nsSNP,其中大多数发生在保护区.
- 预测的潜在的翻译后修饰变化,包括酸化和糖化,在特定的nsSNP位点 (rs669769139,rs666336692).
结论:
- 高血压蛋白中的遗传变异会影响蛋白质功能,可能会影响山羊适应恶劣环境条件.
- 在HSP70A4中的nsSNP rs669769139,一个保存的PTM地点,可能会改变蛋白质的稳定性和功能,影响适应当地气候.
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