的scRNA测序表明了神经板边界细胞命运分配的概率模型
Alexandre P Thiery1, Ailin Leticia Buzzi1, Eva Hamrud1
1Centre for Craniofacial and Regenerative Biology, Faculty of Dentistry, Oral and Craniofacial Sciences, King's College London, London, United Kingdom.
eLife
|August 2, 2023
概括
脊椎动物神经板边界是一个多样化的区域,而不是一个单一的状态. 这个区域内的细胞是未决定的祖先,根据基因表达和位置逐渐采用特定的命运.
科学领域:
- 发展生物学 发展生物学
- 基因组学就是基因组学.
- 细胞生物学 细胞生物学
背景情况:
- 脊椎动物神经板边界 (NPB) 对于形成各种外皮衍生物至关重要.
- 之前在组织层面的研究表明,在NPB中,细胞命运分配的模型是相互矛盾的.
- 了解NPB细胞异质性是解决这些差异的关键.
研究的目的:
- 在神经分泌过程中调查小外皮中的细胞状态多样性和异质性.
- 描述基因模块动力学和模拟外皮命运分离中分子事件的序列.
- 重新评估NPB的性质,作为一个离散的转录状态,而不是一个异质的领土.
主要方法:
- 从原始条纹到神经元的小ectoderm的单细胞RNA测序.
- 对基因模块动态和表达模式的分析.
- 识别和描述"位于边境的未确定祖先" (BLUP).
主要成果:
- 国家皮肤区是一个异质的外皮区域,而不是一个统一的过渡状态.
- 以前被认为是NPB"特征者"的基因表现出动态表达,丰富了特定的命运.
- 单个NPB细胞共同表达竞争的转录程序,表明可塑性.
- 一个"边界位置未确定前代" (BLUPs) 种群逐渐分解成不同的细胞类型.
结论:
- 该NPB包括一个异质的祖先群体与未承诺的细胞命运.
- 在NPB的细胞命运决定是概率性的,受竞争转录程序平衡的影响.
- 祖先的时空定位调节了这些程序的平衡,指导了外皮衍生物的形成.
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