在黄瓜中对介质DNA双链断裂和交叉的全面剖析
Yanling Wang1, Zhaonian Dong1, Yalin Ma2
1Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.
Plant physiology
|August 2, 2023
概括
介质重组形成了作物中的遗传多样性. 这项研究揭示了遗传和表观遗传因素如何影响黄瓜杂交的交叉模式,帮助作物改进策略.
科学领域:
- 植物遗传学 植物遗传学
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- 介质重组对于遗传多样性和作物基因组优化至关重要.
- 了解影响植物杂交物交叉 (CO) 事件的因素是有限的.
- 黄瓜 (Cucumis sativus) 的育种依赖于杂交,以发展精英品种.
研究的目的:
- 调查导致黄瓜中杂交交叉的杂交特异性变化的因素.
- 确定与二氧化碳形成和分布相关的基因组和表观遗传特征.
- 为植物育种中的亲子选择和再组合操纵提供理论支持.
主要方法:
- 从半野生和化黄瓜之间的交叉中构建两个F2种群.
- 检查交叉事件及其在染色体上的分布.
- 分析COs,结构变异 (SVs),序列多态和表观遗传修饰 (CsRAD51A结合,H3K4me3,染色质可访问性,低甲基化) 之间的关联.
主要成果:
- 杂交分布在杂交物之间在染色体上不均地变化,通常在异合性SV区域中被抑制,并通过高序列多态化加速.
- 全球CO景观和本地DNA双链断裂 (DSB) 热点/基因与CsRAD51A结合,H3K4me3,染色体可访问性和低甲基化有积极的关联.
- 在CO事件中,混合体特异性差异与明显的遗传/表观遗传特征,DSB热点和异构性SVs有关.
结论:
- 基因组和表观遗传特征对的交叉形成和杂交特异分歧有显著的贡献.
- 多原子信息可以预测交叉频率和抑制.
- 研究结果为优化父母组合和操纵黄瓜育种计划中的重组提供了理论见解.
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