Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

DNA Base Pairing02:27

DNA Base Pairing

27.5K
Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
27.5K
Base-pairing and DNA Repair02:27

Base-pairing and DNA Repair

64.8K
64.8K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.2K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.2K
DNA as a Genetic Template02:05

DNA as a Genetic Template

22.0K
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
22.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Revisiting the Conformational Flexibility of DNA 3-Arm Junctions for Nanoconstruction.

Nano letters·2026
Same author

Assembly of Protein-DNA Framework Nanostructures: Structurally Defining Protein-DNA Interfaces With Aptamer.

Angewandte Chemie (International ed. in English)·2026
Same author

Cooperativity, entropy, and effective concentration in DNA origami self-replication.

Science advances·2026
Same author

Fluorogenic Aptamer Optimization on a Massively Parallel Sequencing Platform.

ACS sensors·2026
Same author

Conformational equilibria in the activation of cystine-knot hormone receptors.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Programmable Synthesis of DNA Networks in Giant Vesicles via Rolling Circle Amplification.

Nano letters·2026

相关实验视频

Updated: Jul 20, 2025

Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

7.1K

设计3DDNA晶体中的异金属基对编程

Brandon Lu1, Yoel P Ohayon1, Karol Woloszyn1

  • 1Department of Chemistry, New York University, New York, New York 10003, United States.

Journal of the American Chemical Society
|August 2, 2023
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种研究金属介导DNA (mmDNA) 相互作用的新方法. 这种技术精确地捕捉了金属如何在不同的pH值下与DNA基对结合,从而使新的基于DNA的纳米设备成为可能.

更多相关视频

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

20.6K
Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

11.7K

相关实验视频

Last Updated: Jul 20, 2025

Design and Synthesis of a Reconfigurable DNA Accordion Rack
07:44

Design and Synthesis of a Reconfigurable DNA Accordion Rack

Published on: August 15, 2018

7.1K
Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

20.6K
Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

11.7K

科学领域:

  • 生物有机化学
  • 纳米技术
  • 结构生物学

背景情况:

  • 金属介导DNA (mmDNA) 提供了一个将生物无机和电子功能整合到DNA结构的途径.
  • 胺基对之间的可编程金属化是由各种化学和生物物理力量控制的.

研究的目的:

  • 开发一种结晶学方法来捕获不同pH值的DNA中的金属结合模式.
  • 在原子层面阐明金属与DNA相互作用的结构基础.

主要方法:

  • 使用了三维 (3D) DNA 张度三角形图案进行结晶.
  • 使用异常散射技术捕获单金属和多金属结合事件.
  • 在不同的pH条件下确定了28个生物分子结构以分析金属介导的DNA反应.

主要成果:

  • 在T-T和U-U对中观察到银的占用量增加.
  • 在同一个基对中成功捕获了白银 (I) 和 (II),隔离了同金属和异金属结合的定位点.
  • 在高pH下确定C-C对与白银 (I) 和 (II) 的结构,以及T-T对与 (II) 和 (II) 的结构.

结论:

  • 在亚纳米尺度捕获异金属DNA化学的精确方法.
  • 开发的晶体学方法使得基于mmDNA的先进纳米设备的原子设计成为可能.
  • 这项工作为复杂的纳米技术铺平了道路,