全跨网红酸诱导差异化因子 (ATRAID) 的功能性表征
Roya Mehrasa1,2, Ileana Cristea1,3, Cecilie Bredrup1,3
1Department of Clinical Medicine, University of Bergen, Norway.
FEBS open bio
|August 2, 2023
概括
全转网红酸诱导的分化因子 (ATRAID) 异型C因子高度表达和N-糖化. 攻击异形C局部化到细胞膜,囊泡和戈尔吉细胞,与内分体标记物和RAB11.11相互作用.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 蛋白质的表征 蛋白质的表征
背景情况:
- 全跨网红酸诱导分化因子 (ATRAID) 最初在HL60细胞中被发现.
- 存在多个ATRAID的mRNA异型,但它们的蛋白质产物仍未完全表征.
研究的目的:
- 描述ATRAID异型体的蛋白质表达和亚细胞局部化.
- 调查ATRAID异型C的翻译后修改和细胞贩运情况.
主要方法:
- 用Myc-Flag标记的ATRAID异型A,B和C感染细胞.
- 西方涂抹和亚细胞分离以评估蛋白质表达和定位.
- 免疫光显微镜以确定亚细胞分布和与特定标记物共定位.
主要成果:
- 攻击Isoform C表现出高表达,而Isoform A显示出快速降解,而Isoform B是无法检测的.
- 异形C主要以N-糖基化形式被发现.
- 亚细胞分离和免疫光局部化异形C到膜和核分离,细胞质囊泡,戈尔吉区域和内体/溶酶体区.
- ATRAID C 异型体与 LAMP1,LAMP2 和 RAB11 共同局部化,表明与回收内分泌体和膀性贩运途径的关联.
结论:
- 攻击异形C是主要的表达蛋白质产物,其特点是N-糖化和局部化到参与蛋白质贩运的囊泡区.
- 这些发现提供了关于ATRAID C异形的细胞命运和功能的见解,特别是它在内体体贩运途径中的作用.
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