相关实验视频

Updated: Jul 20, 2025

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT
09:06

Enhanced Sample Multiplexing of Tissues Using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: May 1, 2017

7.0K

新型同标签的开发使得使用互补离子的精确和敏感的多重蛋白组学成为可能

Miyang Li1, Min Ma2, Lingjun Li3,4,5

  • 1Department of Chemistry, University of WI-Madison, Madison, WI, 53706, USA.

Analytical and bioanalytical chemistry
|August 2, 2023
PubMed
概括

使用新型二甲基化白补充离子 (DiLeuC) 标签的新方法提供了精确的蛋白质组量化. 这种方法克服了现有方法的局限性,即使在复杂的生物样本中也可以进行敏感分析.

关键词:
补充性离子是补充性离子.这是一个难以理解的难题.异构体标签 (isobaric tags) 是一种同构体标签.质谱测量质量谱测量量化蛋白质组学是一种定量化的蛋白质组学.

更多相关视频

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

12.0K
Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT09:24

Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging cPILOT

Published on: December 18, 2020

5.6K

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.6K
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
6.7K