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在乳鱼,Chanos chanos幼虫的早期本体发生过程中,消化酶活动的变化
Thirugnanamurthy Sivaramakrishnan1,2, Kondusamy Ambasankar3, Nathan Felix4
1TNJFU-Institute of Fisheries Post Graduate Studies (IFPGS), OMR Campus, Vaniyanchavadi, Chennai, 603103, India. sivaraman.fish@gmail.com.
Fish physiology and biochemistry
|August 2, 2023
概括
乳鱼 (Chanos chanos) 幼虫的消化酶活性在化后15-21天之间达到顶峰. 这一时期对于成功地将幼虫从活食中奶到配制料至关重要,优化水产养殖实践.
科学领域:
- 水产养殖和海洋生物学
- 鱼类生理学 鱼类生理学
- 幼虫发展 幼虫发展
背景情况:
- 成功养殖海鱼幼虫需要了解消化系统的发育和营养需求.
- 乳鱼 (Chanos chanos) 水产养殖很重要,但在早期发育过程中最优的养策略需要进一步研究.
研究的目的:
- 为了研究乳鱼幼虫中关键消化酶的本体遗传特征.
- 确定幼虫过渡到配方料的关键发育窗口.
主要方法:
- 对乳鱼幼虫进行了从化后0至30天的酶活性测定 (trypsin, chymotrypsin,leucine aminopeptidase,lipase,amylase,性酸酶).
- 根据标准化的养方案,对幼虫的生长 (长度和体重) 进行监测.
- 酶活性与饮食变化相关,包括活料 (Chlorella,罗提弗,Artemia) 和商业断奶料.
主要成果:
- 试素,胆素和氨酸氨基化酶的特异性活性在化后3天和15天 (dph) 显示出双模峰值.
- 性酸酶和胺酶活动也表现出双模峰值 (3dph和18-21dph).
- 脂酶活性在0,3和18dph时很高,之后出现波动.
结论:
- 乳鱼幼虫的消化酶活性峰值发生在15-21dph之间.
- 这一时期是乳鱼幼虫从活食转移到配方饮食的关键发展窗口.
- 了解这些酶配置文件可以改善幼虫养殖协议和乳鱼化场的料管理.
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