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分子洞察到基因差异化,种群结构和带状图案分析的巴巴拉花生 (Vigna subterranea [L.] Verdc.) 的分子洞察. 与简单序列重复 (ISSR) 相关联

Md Mahmudul Hasan Khan^1,2, Mohd Y Rafii^3,4, Shairul Izan Ramlee⁴

¹Laboratory of Climate-Smart Food Crop Production, Institute of Tropical Agriculture and Food Security (ITAFoS), Universiti Putra Malaysia (UPM), 43400, Serdang, Selangor, Malaysia. mhasan.bari12@gmail.com.

Molecular biology reports

|August 2, 2023

概括

对巴姆巴拉花生加入的遗传分析揭示了重要的人口结构和差异化. 来自Cancaraki和Jatau的加入显示出高度的遗传分歧,这使得它们对未来的育种计划有价值.

科学领域:

农业科学农业科学
遗传学是一个遗传学.
植物育种植物育种

背景情况:

巴姆巴拉花生 (Vigna subterranea [L.] Verdc.) 是一种土壤中生长的花生. 是一个重要的豆类作物.
了解遗传多样性对于作物改善和保护至关重要.

研究的目的:

评估巴马拉地瓜加入中的遗传漂移,人口结构,家族遗传关系和遗传差异化.
为了利用ISSR原料进行分子分析.

主要方法:

采样了来自四个地理区域的11个种群的44个班巴拉花生加入.
用32个ISSR标记器进行基因组DNA放大和分析.
利用分子变异分析 (AMOVA),PhiPT,Nei的遗传距离,遗传树结构和主要坐标分析 (PCoA).

主要成果:

检测到高多态性 (总体97.64%,每个人口35.15%,每个区域51.08%).
AMOVA显示出种群内部 (75%) 和种群之间 (25%),区域内 (84%) 和区域之间 (16%) 的显著差异.
确定了具有独特遗传特征和高遗传距离值的特定加入 (比迪拉利,坎卡拉基,罗科,迈伯戈,卡塔瓦,卡鲁),表明了显著的差异化.

结论:

这项研究突出了班巴拉花生种群中的遗传多样性,这对育种计划至关重要.
由于高度的遗传分歧和同卵性,建议从坎卡拉基和贾陶加入以后的繁殖.
这些发现支持加强对现有的巴马拉地瓜遗传资源的利用和保护.

关键词:

带带的图案带带的图案遗传多样性,NJ和N-网络化遗传学链接遗传学链接人口结构人口结构维格纳地下 [L.] Verdc. 地下维格纳

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