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在低温电子显微镜中充电玻璃样本,用石墨烯缓解

Yue Zhang¹, J Paul van Schayck¹, Adrián Pedrazo-Tardajos^2,3

¹Maastricht MultiModal Molecular Imaging Institute (M4i), Maastricht University, 6200 MD Maastricht, The Netherlands.

|August 2, 2023

概括

将石墨烯层添加到电子显微镜网格中,可以显著减少样本的充电,从而实现更高分辨率的宏分子重建. 这一进步提高了数据质量,并为先进的冷电磁技术开辟了新的可能性.

科学领域:

结构生物学结构生物学
材料科学材料科学材料科学
显微镜的使用方法

背景情况:

低温电子显微镜 (cryo-EM) 可以实现高分辨率的宏分子重建,但分辨率受到光束诱导的运动和图像扭曲的限制.
样本充电是导致这些文物的一个关键因素,通常通过将支膜纳入光束路径来管理.

研究的目的:

在低温电子显微镜 (cryo-EM) 过程中对玻璃样本的静电充电进行表征.
研究石墨烯作为一种解决方案,用于缓解冷EM中的样本充电器件.
通过减少光束诱导的样本移动来提高单粒子分析 (SPA) 的分辨率极限.

主要方法:

在成像和衍射模式中对静电充电的表征.
在标准EM网格上沉积一层导电石墨烯.
高分辨率单粒子分析 (SPA) 数据收集在带有聚焦束辐射的石墨烯涂层网格上.

主要成果:

石墨烯涂层有效地减轻了样本的充电,即使电子束避免了与支膜的直接接触.
使用数据收集策略实现了2 Å分辨率的SPA重建,这些数据收集策略以前产生了3 Å分辨率.
与未涂层网格相比,采用石墨烯涂层网格的SPA数据显示B因子得到改善,颗粒运动减少.

结论:

涂上石墨烯的网格提供了一种切实可行的方法来减少冷EM中的充电器件,提高分辨率和数据质量.
这种方法对SPA,密度图,辐射损伤研究以及未来的EM样本载体具有广泛的影响.
促进了先进的,剂量高效的冷电磁技术的探索,包括扫描传输基于EM的SPA.

关键词:

收费收费收费收费低温传输电子显微镜电子显微镜石墨烯是一种石墨烯.一个粒子分析分析.玻璃体样本的样本.

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