一个新型的基因型2猪肠病毒G的表征,遗传学分析和病原性
Dai Xiao1, Luwen Zhang1, Shiqian Li1
1Research Center for Swine Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.
Virus research
|August 2, 2023
概括
在中国发现了一种新的肠道病毒G基因型2菌株 (EV-G2),CHN-SCMY2021,在中国发现了一种新的肠道病毒G基因型2菌株 (EV-G2). 这种病毒引起小猪轻度腹,并影响多个器官,扩大了对EV-G流行病学和进化的知识.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子流行病学分子流行病学
- 动物健康 动物健康
背景情况:
- 肠道病毒G (EV-G) 是与各种动物疾病相关的皮科纳病毒.
- 在全球范围内,对EV-G菌株,特别是基因型2的有限表征存在.
- 了解新型EV-G菌株对于动物疾病监测和控制至关重要.
研究的目的:
- 来自中国的新型EV-G基因型2菌株的隔离和特征.
- 调查新菌株的基因组特征,细胞热带性和病原性.
- 为了解EV-G流行病学和分子进化做出贡献.
主要方法:
- 对新型EV-G2菌株CHN-SCMY2021的隔离和全基因组测序.
- 基于VP1序列和全基因组比较的遗传学分析.
- 重组分析以确定潜在的母菌株.
- 在体外细胞培养适应性研究.
- 在体内对小猪的致病性评估.
主要成果:
- 在中国,首个EV-G基因型2菌株CHN-SCMY2021被分离和鉴定.
- 遗传学分析证实其被归类为EV-G基因型2,与已知菌株的全基因相似性为78.3-86.4%.
- 重组分析表明,来自KT265894.2和AF363455.1.1菌株的混合起源.
- CHN-SCMY2021适应多个细胞系 (BHK-21,PK-15,IPEC-J2,LLC-PK,Vero),但不是ST细胞.
- 在小猪中,该病毒引起轻度腹,肠壁变薄,并传播到多个器官,包括肠道,心脏,肝脏,脏,肺部,脏,大脑和脊髓.
结论:
- CHN-SCMY2021代表了中国第一个系统地表征的EV-G基因型2菌株.
- 这一发现扩大了EV-G.的已知地理分布和遗传多样性.
- 这项研究为EV-G基因型2的分子进化,致病性和宿主组织热带性提供了宝贵的见解.
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