相关实验视频

Updated: Jul 20, 2025

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies

Published on: January 31, 2025

来自结性脊柱炎患者的血外体的蛋白质和基因组分析

Fataneh Tavasolian¹, Starlee Lively^1,2, Chiara Pastrello^1,2,3

¹Schroeder Arthritis Institute, Toronto Western Hospital, University Health Network, Toronto, Ontario, Canada.

Annals of the rheumatic diseases

|August 2, 2023

概括

脊髓灰质炎 (AS) 患者表现出明显的外体样本,具有改变的microRNA (miRNA) 和蛋白质表达,有助于免疫失调. 来自AS的外体抑制了调节性T细胞的增殖,提供了对疾病病原和潜在治疗点的见解.

科学领域:

免疫学免疫学免疫学
基因组学就是基因组学.
蛋白质组学是指蛋白质组学.
异构体生物学异构体生物学

背景情况:

脊髓灰质炎 (AS) 的发病包括免疫失调,由改变的基因,microRNA (miRNA) 和蛋白质表达的潜在贡献.
外基因组通过miRNA运输参与细胞间通信,涉及到延续AS的炎症.

研究的目的:

在AS患者中进行第一个血衍生外体的蛋白质和miRNA分析.
研究AS衍生的外体对CD4+T细胞,特别是调控性T细胞 (Tregs) 的功能影响.

主要方法:

从AS患者和健康对照组 (HC) 中分离和表征血衍生的外体,使用超离心和流细胞计.
对外体表面标记物和细胞因子概况的多重免疫捕获试验.
下一代测序用于miRNA识别和计算生物学分析.
与AS外生体和HC CD4+ T细胞进行共同培养实验,以评估T细胞子集的频率和增殖.

主要成果:

与HC患者相比,AS患者的外体标记CD63和CD81的表达增加.
在AS衍生的外体中降低IL-8和IL-10的调节;在与HC CD4+ T细胞共同培养时,IFNα2和IL-33的上调调节.
亚氏体外体抑制了FOXP3+调节性T细胞 (Tregs) 的增殖.
微RNA测序确定了AS外体中24个差异表达的微RNA,其中22个是上调的,2个是下调的.

结论:

患有AS的个体具有独特的基于外体的免疫学和遗传特征.
在AS中Treg增殖的外体介导抑制表明有助于该疾病中T细胞激活的机制.

关键词:

结性脊柱炎是一种疾病.关节炎是一种关节炎.自免疫性疾病自免疫性疾病这是一种自身免疫力.

更多相关视频

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Published on: May 27, 2016

相关概念视频

Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...