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UGT2B13和UGT2C1涉及罗帕洛西姆 (Rhopalosiphum padi) 中的兰巴-甲素耐药性

Suji Wang¹, Xi Liu¹, Hongcheng Tang¹

¹State Key Laboratory of Crop Stress Biology for Arid Areas, Key laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi, China.

Pesticide biochemistry and physiology

|August 2, 2023

概括

尿素二酸-葡萄糖转移酶 (UGTs) 在小麦害虫Rhopalosiphum padi中对排毒杀虫剂至关重要. 过度表达的UGT2B13和UGT2C1基因显著促进了这种虫物种的兰巴-甲素耐药性.

科学领域:

* 昆虫学昆虫学
* 分子生物学 * 分子生物学
* 生物化学 * 生物化学

背景情况:

*尿素二酸盐-葡萄糖转移酶 (UGTs) 是关键的第二阶段解毒酶.
* 它们在抗杀虫剂,特别是农业害虫的耐药性方面的具体作用,在很大程度上仍未得到阐明.
*罗帕洛西姆 (Rhopalosiphum padi) 是全球重要的小麦害虫,对经济构成重大威胁.

研究的目的:

* 为了识别和描述Rhopalosiphum padi.中的UGT基因.
* 调查特定的UGTs (UGT2B13和UGT2C1) 在兰巴基素耐药性的参与.
*阐明这些UGT在杀虫剂代谢和耐药机制中的功能作用.

主要方法:

* 在Rhopalosiphum padi中识别了UGT基因 (UGT2B13,UGT2C1).
*生物测试以确定对兰巴-甲的耐药性比率.
*对敏感和耐药菌株的基因表达分析 (量化和转录水平).
*RNA干扰 (RNAi) 来评估UGT敲除对昆虫杀虫剂敏感性的功能影响.

主要成果:

* 在R. padi耐药菌株 (LC-R) 中观察到对lambda-cyhalothrin的高耐药比率 (2963.8倍).
* UGT基因表达和含量在LC-R菌株中显著增加,特别是在肠道中.
*通过RNAi介导的UGT2B13或UGT2C1的沉默降低了UGT水平,并在暴露于lambda-cyhalothrin时增加了虫死亡率.

结论:

* UGT2B13和UGT2C1在Rhopalosiphum padi.中赋予兰巴-cyhalothrin耐药性的过程中发挥着重要作用.
*这些UGT参与了lambda-cyhalothrin的代谢排毒,有助于抗昆虫剂.
* 调查结果强调UGTs作为管理这种主要农业害虫的抗昆虫剂耐药性的潜在目标.

关键词:

兰巴达-甲氨酸三素这是一种RNA干扰.罗帕洛西姆可能会出现.尿素二酸盐 - 葡萄糖转移酶

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