利蒙的抗TMV活性及其作用方式
Wei Luo1, Kaiyue Wang1, Jingyi Luo1
1College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China; Provincial Center for Bio-Pesticide Engineering, Yangling, Shaanxi 712100, China.
Pesticide biochemistry and physiology
|August 2, 2023
概括
皮油中的烯通过激活植物免疫反应,对烟草马赛克病毒 (TMV) 具有强烈的抗病毒活性. 这种天然化合物还可以保护其他植物病原体,表明其作为植物衍生农业剂的潜力.
科学领域:
- 植物病理学 植物病理学
- 生物化学 生物化学
- 农业科学 农业科学
背景情况:
- 烯是皮油中的单烯,具有抗菌,杀菌和抗瘤的特性.
- 它的农业发展价值是显著的,特别是在植物保护方面.
研究的目的:
- 评估利蒙对烟草马赛克病毒 (TMV) 的生物活性.
- 为了研究烯诱导的植物免疫的潜在机制.
主要方法:
- 评估了利蒙对TMV的保护,无活化和治愈作用.
- 确定了利蒙的诱导活性和对烟草过敏反应 (HR) 的影响.
- 分析了生理和生化变化,包括防御酶活性,PR基因表达,酸 (SA) 积累和SA相关的基因表达.
主要成果:
- (800μg/mL) 显示出高的TMV保护 (84.93%),无活化 (59.28%) 和治疗 (58.89%) 活动.
- 利蒙触发了HR,并诱导了防御酶 (SOD,CAT,POD,PAL) 和PR基因 (NPR1,PR1,PR5).
- 利蒙增加了SA积累和相关基因表达 (PAL,RBOHB),表明SA介导的全身性获得性耐药性 (SAR).
- 利蒙还增强了叶绿素生物合成基因 (POR1) 表达,并提供了对Sclerotinia sclerotiorum,Phytophthora capsici和Botrytis cinerea的抵抗力.
结论:
- 利蒙对植物病原体具有强大的广泛抗病毒和抗微生物活性.
- 它通过SA信号通路激活植物免疫力,诱导SAR.
- 利蒙是一种有前途的植物性衍生剂,用于农业应用,作为抗病毒剂,免疫激活剂,杀虫剂和杀菌剂.
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