探测N端乙化对α-synuclein结构,聚合和细胞毒性的影响
Rosie Bell1, Michele Vendruscolo1, Janet R Kumita2
1Yusef Hamied Department of Chemistry, University of Cambridge, Cambridge, United Kingdom.
Methods in enzymology
|August 2, 2023
概括
研究了N-终端乙化α-synuclein (NTA α-synuclein) 聚合,以了解帕金森病. 开发了分析NTAα-synuclein聚合动力学和细胞效应的方法.
科学领域:
- 神经科学是一个神经科学.
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 帕金森病涉及到大脑细胞中异常的α-synuclein聚合.
- 阿尔法同核素的翻译后修改与其聚合有关.
- N-终端乙化是一种常见的α-synuclein的修饰.
研究的目的:
- 描述生产和分析N-终端乙化 (NTA) α-synuclein的方法.
- 在体外和细胞系统中研究NTAα-synuclein的聚合行为.
- 为研究NTAα-synuclein聚合对细胞功能的影响提供协议.
主要方法:
- 生产N-终端乙化 (NTA) 的α-synuclein.
- 用于聚合测试的NTAα-synuclein种子的制备.
- 对NTAα-synuclein聚合的动态分析.
- 基于细胞的测试使用预制的NTAα-synuclein原纤维素.
主要成果:
- 建立了NTAα-synuclein生产和聚合分析的协议.
- 关于NTAα-synuclein聚合的动态分析的详细方法.
- 概述了基于细胞的测试,以评估NTAα-synuclein原纤维素的影响.
结论:
- 描述的方法允许详细研究NTAα-synuclein聚合.
- 这些协议可以调整以研究其他α-synuclein修饰.
- 该方法适用于研究其他容易聚合的蛋白质中的N-乙化.
相关概念视频
Phase II Reactions: Acetylation Reactions
268
Acetylation, a phase II biotransformation reaction, introduces an acetyl group to drugs or their metabolites. Acetyltransferase enzymes facilitate this reaction, which resembles α-amino acid conjugation due to the addition of a functional group to the drug molecule.
The substrates for acetylation are typically drugs or their metabolites with an amino, sulfonamide, or hydrazine functional group. Acetylation can occur at several points in the drug molecule, including primary, secondary, and...
The substrates for acetylation are typically drugs or their metabolites with an amino, sulfonamide, or hydrazine functional group. Acetylation can occur at several points in the drug molecule, including primary, secondary, and...
268
Amyloid Fibrils
9.6K
Amyloid fibrils are aggregates of misfolded proteins. Under most circumstances, misfolded proteins are either refolded by chaperone proteins or degraded by the proteasome. However, in the case of a mutation or a disease, these proteins can accumulate to form large clusters and often further assemble to form elongated fibers, called fibrils.
Amyloid deposits were observed as early as 1639 in the liver and the spleen. In 1854, Rudolph Virchow performed iodine staining,...
Amyloid deposits were observed as early as 1639 in the liver and the spleen. In 1854, Rudolph Virchow performed iodine staining,...
9.6K
Amines to Amides: Acylation of Amines
2.5K
Various carboxylic acid derivatives (such as acid chlorides, esters, and anhydrides) can be used for the acylation of amines to yield amides. The reaction requires two equivalents of amines. The first amine molecule functions as a nucleophile and attacks the carbonyl carbon to produce a tetrahedral intermediate. This is followed by the loss of the leaving group and restoration of the C=O bond.
Next, the second equivalent of amine serves as a Brønsted base and deprotonates the quaternary...
Next, the second equivalent of amine serves as a Brønsted base and deprotonates the quaternary...
2.5K


