在农药治疗后,Leptinotarsa decemlineata幼虫的生存是否取决于微生物组的组成?
Jitka Stara1, Jan Hubert1,2
1Crop Research Institute, Prague, Czechia.
Pest management science
|August 2, 2023
概括
科罗拉多土豆虫的微生物群,特别是乳菌,有助于对抗农药pyrifos的生存. 用L. lactis补充幼虫显著降低了死亡率,突出显示了它在抗农药耐药性方面的作用.
科学领域:
- 微生物学 微生物学
- 昆虫科学 昆虫科学 昆虫科学
- 农药耐药性 农药耐药性是一种
背景情况:
- 关节动物微生物组可以排毒农药或提高宿主免疫力.
- 科罗拉多马甲虫 (CPB; Leptinotarsa decemlineata) 是一种主要的农业害虫,以抗农药性而闻名.
- 了解CPB微生物组的动态对于害虫管理策略至关重要.
研究的目的:
- 分析不同地理位置的CPB幼虫微生物组组成.
- 研究pyrifos农药对存活和死亡幼虫的微生物群的影响.
- 确定Lactococcus lactis在CPB农药耐药性中的作用.
主要方法:
- 16S rRNA基因测序以表征幼虫微生物组.
- 实验室实验涉及对CPB幼虫进行pyrifos处理.
- 操纵实验以评估L. lactis补充剂对幼虫死亡率的影响.
主要成果:
- CPB幼虫微生物组包括少数操作分类单元 (OTU),包括Enterobacteriaceae,Pseudocitrobacter,Acinetobacter,Pseudomonas,L. lactis,Enterococcus,Burkholderia,以及Spiroplasma leptinotarsae. 这三种类型的细菌包括:
- 微生物组组成因采样地点而异,并在甲酸暴露后的生存和死亡幼虫之间存在差异.
- 较高比例的L. lactis序列与幼虫存活率相关;L. lactis补充剂使死亡率降低了五倍.
结论:
- CPB幼虫微生物组的组成受样本取样地点的影响.
- 肠道共生体Lactococcus lactis在缓解CPB幼虫中pyrifos的毒性作用方面发挥着重要作用.
- 准L. lactis可能为管理这种农业害虫的农药耐药性提供了一种新方法.
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