相关实验视频
Updated: Jul 20, 2025

10:37
Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
35.8K
尼特林-1的药理向抑制了癌症中的EMT
Justine Lengrand1,2,3, Ievgenia Pastushenko1, Sebastiaan Vanuytven4,5
1Laboratory of Stem Cells and Cancer, Université Libre de Bruxelles (ULB), Brussels, Belgium.
Nature
|August 2, 2023
概括
在皮肤癌中,用NP137抗体向netrin-1可抑制表皮细胞转化为介质细胞转化 (EMT). 这种方法减少了转移,提高了化疗的敏感性,为癌症治疗提供了新的治疗策略.
科学领域:
- 癌症学
- 分子生物学
- 癌症研究
背景情况:
- 表皮细胞转移到介质细胞转移 (EMT) 对瘤的开始,进展,转移和治疗耐药性至关重要.
- 尽管了解EMT的作用,但尚未确定有效的治疗策略.
研究的目的:
- 研究网林-1在皮肤状细胞癌 (SCC) 中调节EMT的作用.
- 评估使用单克隆抗体NP137抑制癌症EMT的治疗潜力.
主要方法:
- 使用皮肤SCC的主要小鼠模型与自发EMT.
- 进行NP137抗体的治疗,并评估其对EMT,转移和化疗敏感性的影响.
- 使用单细胞RNA测序来分析EMT状态.
- 在试验室中对网林-1及其受体UNC5B进行了敲击.
- 在接受EMT的人类A549肺癌细胞移植的小鼠中测试了NP137的疗效.
主要成果:
- 在皮肤SCC表现出EMT的小鼠模型中,Netrin-1的调节升高.
- 在皮肤SCC中,NP137治疗减少了EMT瘤细胞,减少了转移,增加了化疗敏感性.
- NP137预防了晚期EMT状态的进展,并维持了上皮瘤状态.
- 在实验室中,Netrin-1 抑制了EMT,并调节了促进上皮状况的基因特征.
- 在人类A549肺癌细胞中,NP137降低了EMT.
结论:
- 在癌症中,Netrin-1 在调节EMT方面发挥着重要作用.
- 使用NP137的药理抑制是针对各种癌症使用EMT的有希望的治疗策略.
- 这种方法提供了新的治疗干预措施来对抗癌症的进展,并提高治疗效率.
相关概念视频
Mitogens and the Cell Cycle
6.6K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.6K
Metastasis
5.6K
Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
5.6K
Cadherins in Tissue Organization
3.0K
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
Cell Sorting During Development
Cell sorting plays an...
3.0K
Targeted Cancer Therapies
7.7K
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
There are several types of targeted therapies against...
7.7K
Cancer Cell Migration through Invadopodia
2.3K
Invadosome is a broad category of cell surface structures with proteolytic activity that degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
2.3K
Non-Canonical Wnt Signaling Pathways
7.3K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.3K

