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在Setaria viridis中,YABBY基因SHATTERING1控制了割区域的激活,而不是该区域的模式

Yunqing Yu¹, Hao Hu², Daniel F Voytas³

¹Donald Danforth Plant Science Center, 975 North Warson Road, St Louis, MO, 63132, USA.

The New phytologist

|August 2, 2023

概括

在Setaria viridis中,SHATTERING1 (SH1) 通过控制素信号传递和分离区域的叶绿体退化来调节植物的碎裂. 这与它在米中的作用不同,突出了这种化基因的多样化功能.

科学领域:

植物生物学植物生物学
遗传学遗传学是一个
分子生物学分子生物学

背景情况:

剥离是植物的一个关键的发育过程,由专门的剥离区 (AZ) 调节.
一个YABBY转录因子SHATTERING1 (SH1) 是一个关键的化基因,控制了米类和Setaria等谷物中的破碎.
米中的SH1的功能包括抑制AZ化,但其在非化AZs的Setaria中的作用尚不清楚.

研究的目的:

调查SH1在Setaria viridis中的功能,特别是在具有非线性脱离区域的物种中.
阐明SH1调节Setaria中脱离的分子机制.

主要方法:

在Setaria viridis中为SH1生成CRISPR-Cas9淘汰突变物.
组织学分析,细胞壁成分分析和免疫光检测辅酶.
传输电子显微镜,激素治疗和RNA-Seq分析.

主要成果:

sh1突变体表现出缺乏破碎,证实了sh1在这个过程中的作用.
在WT和sh1突变之间没有观察到细胞解剖或素的显著差异,直到脱离.
在WT的AZ中,SH1对于叶绿体退化至关重要,这一过程受到auxin信号传递和分布的影响.

结论:

在Setaria viridis中,SH1通过辅酶信号激活了脱离,这与它在中的作用不同.
这项研究揭示了SH1在调节在草中脱离过程中的叶绿体退化和辅酶通路方面的新功能.

关键词:

破碎1 破碎1 破碎1 这种病原体是Setaria viridis.哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈哈切除区域是切除区域.辅助剂辅助剂辅助剂类塑料是一种类塑料.

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