相关实验视频
Updated: Jul 20, 2025

09:47
AFM and Microrheology in the Zebrafish Embryo Yolk Cell
Published on: November 29, 2017
8.3K
在E-YSL中,阿克丁介导的内细胞分裂有助于驱动斑马鱼的表皮细胞
Jackie C Cheng1, Andrew L Miller1, Sarah E Webb1
1The Division of Life Science and Key State Laboratory for Molecular Neuroscience, HKUST, Clear Water Bay, Hong Kong, China.
概括
斑马鱼胚胎在表皮生殖过程中表现出外表黄膜同胞层 (E-YSL) 的动态膜变化. 动蛋白带和类似微的结构对于内细胞分裂至关重要,驱动胚胎发育.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 斑马鱼的胚胎发生
背景情况:
- 斑马鱼胚胎中的外部黄膜同胞层 (E-YSL) 在表皮生殖过程中发挥着关键作用.
- 在E-YSL中形成一条点状F-actin带,在后期的表.
研究的目的:
- 为了研究斑马鱼表期间E-YSL膜的动态变化.
- 阐明F-actin带和膜结构在E-YSL功能中的作用.
主要方法:
- 扫描和传输电子显微镜 (EM) 用于超结构分析.
- 光共聚焦显微镜可视化F-actin的分布.
- 免疫黄金标签用于定位actin集群.
- 用细胞素B进行药理治疗.
主要成果:
- E-YSL表面呈现出绕的微型状突出,在表过程中收窄.
- 动蛋白集群位于这些突起中,并在E-YSL中更深处.
- 斑点性动因带与活跃内细胞分裂的区域一致.
- 细胞素B破坏了actin结构,突起,并减轻了内细胞分裂.
结论:
- 膜突起和相关的动蛋白带是E-YSL局部内细胞形成的组成部分.
- 这一过程对于斑马鱼胚胎中表皮球的进展至关重要.
- 动因动力学和膜重塑是胚胎发育的关键调节者.
更多相关视频
09:39Live Imaging of Cell Extrusion from the Epidermis of Developing Zebrafish
Published on: June 27, 2011
14.9K
08:33Author Spotlight: Strategies for Mounting Zebrafish Embryos for High-Resolution Multiview Light-Sheet Microscopy — Techniques for Imaging and Image Reconstruction
Published on: July 19, 2024
842
相关概念视频
Gastrulation
57.6K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
57.6K
Cell Motility through Blebbing
1.9K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Blebbing Through the Matrix
In multicellular...
1.9K
Cleavage and Blastulation
45.3K
After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
45.3K