这是从长角甲虫基因组中获得的水平获得的GH-7细胞基因酶的第一个证据
1Department of Insect Symbiosis, Max Planck Institute for Chemical Ecology, Jena, Germany.
Archives of insect biochemistry and physiology
|August 3, 2023
概括
长角甲虫拥有独特的酶 (GH7),通过横向基因转移从真菌中获得. 这些酶有效地分解纤维素,有助于这些木食昆虫的消化.
科学领域:
- 生物化学 生物化学
- 进化生物学 进化生物学
- 昆虫学 昆虫学是一门学科.
背景情况:
- 食昆虫,如长角甲虫,依赖于碳水化合物活性酶 (CAZymes) 来消化植物多糖.
- 糖化酶家族7 (GH7) 酶对于碳水化合物分解至关重要,但昆虫编码的GH7很少见.
研究的目的:
- 研究昆虫编码的GH7酶在长角甲虫Exocentrus adspersus中的功能和进化起源.
- 为了确定已识别的GH7酶的基质特异性和活性.
主要方法:
- 基因组分析以确定E. adspersus.sus.中的GH7基因.
- 遗传学分析以推断GH7基因的进化历史.
- 再组合蛋白表达和酶分析以确定酶功能.
主要成果:
- 在E. adspersus基因组中发现了两个并列的GH7基因,并列着可转移的元素.
- 遗传学分析表明,GH7基因的水平基因转移 (HGT) 从真菌转移到E.adspersus.
- 复合E.adspersus GH7酶作为细胞氧化酶起作用,将纤维素分解为纤维素,其活性由化增强.
结论:
- 该研究确定了第一个昆虫编码的GH7酶,可能通过HGT从真菌中获得.
- 这些GH7纤维化水解酶与其他酶一起,使E.adspersus.能够有效地降解纤维素.
- 这些发现突显了Metazoa中GH7酶在多糖化物消化中的独立招募.
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