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吸烟暴露会破坏牙纸干细胞的再生潜力

Leyla Tahrani Hardin¹, David Vang¹, Der Thor¹

¹Department of Biomedical Sciences, Arthur A. Dugoni School of Dentistry, University of the Pacific, San Francisco, United States.

Tobacco induced diseases

|August 3, 2023

概括

香烟烟雾对牙髓干细胞 (DPSC) 产生负面影响,减少它们的生存,迁移和改变细胞因子配置. 这种损伤会影响再生潜力,突出显示吸烟者需要个性化干细胞疗法.

科学领域:

干细胞生物学干细胞生物学
再生医学是一种再生医学.
毒理学毒理学毒理学

背景情况:

吸烟会对中酶体干细胞 (MSCs) 的特性产生不利影响.
研究香烟烟雾对牙髓干细胞 (DPSC) 的影响对于了解其对再生潜力的影响至关重要.

研究的目的:

评估暴露于香烟烟雾对DPSC再生特性的影响.
为了确定香烟烟雾凝液 (CSC) 和尼古丁对DPSCs的剂量依赖影响.

主要方法:

DPSCs被用不同剂量的CSC和尼古丁治疗.
评估了细胞增殖,存活,迁移,细胞因子表达和突变性.
通过ELISA和qPCR分析了信号通路 (AKT,ERK) 和基因表达.

主要成果:

较低的CSC/尼古丁剂量没有影响增殖,但较高的剂量减少了增殖.
DPSC存活率和迁移受到了剂量依赖的损害.
细胞因子概况发生变化,IL-6,TNF-α,IL-10增加,TGF-β1.1.改变.
CSC诱导的突变性高于尼古丁;尼古丁增加了AKT/ERK酸化.

结论:

暴露在香烟烟雾中显著损害了DPSC的再生能力.
吸烟引起的DPSC损伤的分子机制需要进一步研究.
研究结果可能会为吸烟患者提供个性化的干细胞治疗.

关键词:

提供服务的DPSCs.这是一种炎症炎症炎症炎症.尼古丁是一种尼古丁.复兴再生是一种再生方式.吸烟是为了吸烟.

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