рибофлавин化合物显示NAD(P) H依赖的氨酸氧降解酶类氨酸降解活性
Midori Nagase1, Miku Sakamoto1, Sakiko Amekura1
1School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura-cho, Hachioji, Tokyo 192-0982, Japan.
Journal of clinical biochemistry and nutrition
|August 3, 2023
概括
研究人员发现,里波弗拉,而不是NQO1,减少了老鼠肝脏中的辅酶Q10. 这种依赖黄素的途径可能对细胞液体中抗氧化剂防御至关重要.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 氧化压力研究研究 氧化压力研究
背景情况:
- 依赖NAD (P) H的子氧化还原酶 (NQO) 对于对抗氧化应激的细胞保护至关重要.
- NQO酶利用NAD (P) H作为电子供体,促进了辅酶Q (CoQ) 的降解.
研究的目的:
- 为了调查辅酶Q10在老鼠肝脏同质化中的降低活性的来源.
- 为了区分活性与已知的NQO酶,并确定负责的化合物.
主要方法:
- 鼠肝同质化的凝过色谱. 鼠肝同质化的凝过色谱.
- 使用迪库马罗和奎尔塞丁进行酶抑制测定.
- 活性化合物的净化和鉴定.
- 在CoQ和维生素K同类物质上测试已识别的化合物的降解活性.
主要成果:
- 在高分子量 (NQO) 和低分子量分量中发现了辅酶Q10的降解活性.
- 低分子量活性没有被迪库马罗抑制,但被奎尔塞丁抑制.
- 鉴定出依赖于NADH的CoQ10降低是通过 рибофлавин调解的.
- riboflavin, FAD 和 FMN 与 NADH 结合,降低了 CoQ 和维生素 K 的同类物质.
结论:
- 利博弗拉,而不是NQO1,是大鼠肝中NADH依赖的CoQ10减少的重要部分.
- 这种依赖于黄素的降解机制,可能涉及素B,可能在水性细胞区中起作用.
- 这些发现表明,在生物系统中,抗氧化剂防御和CoQ减少的新途径.
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