葡萄糖通过增加二细胞阶段的脂质合成来促进早期胚胎发育
Mingwen Wang1, Jinfeng Xue1, Chanyi Li1
1Reproductive Medical Center, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.
Frontiers in cell and developmental biology
|August 3, 2023
概括
将葡萄糖添加到体外受精 (IVF) 培养介质中可以显著改善早期胚胎发育,特别是通过通过增强的脂质生物合成克服2细胞阻断.
科学领域:
- 生殖生物学 生殖生物学
- 发展生物学 发展生物学
- 代谢学 代谢学 代谢学
背景情况:
- 优化培养条件对于改善体外受精 (IVF) 胚胎发育至关重要.
- 葡萄糖在早期胚胎微环境中的作用仍然不清楚,葡萄糖是卵巢液中存在的必要碳来源.
- 早期分裂阶段 (2 细胞,4 细胞,8 细胞) 呈现出独特的代谢特征,对发育至关重要.
研究的目的:
- 研究不同葡萄糖度对小鼠胚胎发育和新陈代谢的影响.
- 阐明葡萄糖影响早期胚胎发育和克服发育障碍的潜在分子机制.
主要方法:
- 液体染色学-质谱学 (LC-MS) 用于分析不同裂解阶段的培养基中的代谢物.
- 胚胎发育率在从0到5mmol/L的葡萄糖度下进行了评估.
- 转录组分析和尼罗河红色染色被用来研究基因表达和脂质积累.
主要成果:
- 对于2细胞和8细胞阶段,确定了不同的代谢概况,而4细胞阶段作为过渡.
- 用3mmol/L葡萄糖补充剂显著增加了胚胎发育率,从9.95%增加到87.33%,主要克服了2细胞块.
- 在2细胞阶段,葡萄糖上调脂质生物合成基因表达,由增加的脂质积累证实.
结论:
- 葡萄糖代谢在胚胎早期发育中起着至关重要的作用.
- 在IVF介质中补充葡萄糖可以通过促进脂质生物合成和克服2细胞阻断来提高胚胎发育能力.
- 培养介质的代谢物分析有可能用于估计IVF中的胚胎质量.
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