在共生体被淘汰后,脂质代谢功能障碍与改变的肯尼迪通路恒温有关
Geoffrey M Attardo1,2, Joshua B Benoit1,3, Veronika Michalkova1,4
1Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA.
iScience
|August 3, 2023
概括
飞的怀孕依赖于由共生细菌调节的脂质代谢. 破坏这种共生或关键的脂质合成基因会导致母亲的肥胖和后代的饥饿,突出显示了Wigglesworthia细菌的重要作用.
科学领域:
- 昆虫的繁殖 昆虫的繁殖
- 这种共生是共生.
- 脂质代谢 脂质的代谢
背景情况:
- 脂质新陈代谢对于昆虫的繁殖至关重要,特别是在具有大量父母投资的物种中.
- 在昆虫怀孕期间,共生细菌在母体脂质代谢中的作用尚不清楚.
研究的目的:
- 为了研究脂质代谢和在飞 (Glossina morsitans morsitans) 怀孕期间的义务内生 Wigglesworthia glossinidia 之间的相互作用.
- 阐明Wigglesworthia影响母体脂质稳态和生殖成功的分子机制.
主要方法:
- 在 tsetse 怀孕期间对 CTP: 胆酸基因转移酶 (cct1) 的基因表达分析.
- 试验性去除Wigglesworthia共生体,以评估对脂质代谢的影响.
- 在缺乏共生体的中对母体健康和后代发育的表型分析.
- 实验性抑制cct1以验证其在观察到的表型中的作用.
主要成果:
- 策的怀孕与通过肯尼迪通路的酸丁胆生物合成中的关键酶cct1的表达增加有关.
- 实验性消除Wigglesworthia破坏了肯尼迪通路,导致孕产妇肥胖和胚胎饥饿.
- 抑制cct1模仿了在非共生 (无Wigglesworthia) 中观察到的表型,证实了它的关键作用.
结论:
- 共生性细菌,特别是Wigglesworthia,对于维持孕的正常脂质代谢至关重要.
- 交生体衍生因素,可能是B族维生素,对于调节脂质生物合成和脂解都至关重要,从而确保了飞的生育能力.
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