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LRRC15的表达表明,中酶体干细胞中由TWIST1调节的高水平的干性
Kensuke Toriumi1, Yuta Onodera2, Toshiyuki Takehara2
1Department of Orthopedic Surgery, Kindai University Faculty of Medicine, Osaka-sayama, Osaka, Japan.
iScience
|August 3, 2023
概括
研究人员将LRRC15确定为评估间酶体干细胞 (MSC) 质量的可靠标记物. 这种跨膜蛋白有助于预测TWIST1调节的干性,这对于有效的细胞疗法应用至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 再生医学是一种再生医学.
- 生物技术是生物技术.
背景情况:
- 介质干细胞 (MSC) 对于细胞疗法至关重要,但缺乏可靠的质量评估方法.
- 评估MSC的治疗潜力是一项挑战,阻碍了更广泛的临床应用.
- TWIST1是一个关键的转录因子,调节MSC的干度.
研究的目的:
- 确定可靠的标记物来评估MSC的干度和治疗质量.
- 调查TWIST1和细胞表面标记物之间的相关性.
- 在临床前模型中验证新型标记物的实用性.
主要方法:
- 在MSC中分析TWIST1表达的分析.
- 识别和验证LRRC15作为与TWIST1.1相关的标志物.
- 从人类和小鼠骨髓中分离和描述LRRC15阳性MSC种群.
- 在孤立的MSC中评估干性标记物和治疗性细胞因子.
- 在白胺诱导的肺纤维化小鼠模型中评估治疗疗效.
主要成果:
- LRRC15的表达与TWIST1有很强的相关性,TWIST1是MSC茎度的调节者.
- 具有LRRC15阳性的MSCs表现出较高的干性因子和治疗性细胞因子的表达.
- 有LRRC15阳性的MSC在肺纤维化模型中表现出增强的治疗效果.
- LRRC15作为MSC干度和质量的预测标记.
结论:
- TWIST1在维护MSC茎状性方面发挥着至关重要的作用.
- LRRC15是一种有价值的细胞表面标记物,用于预测MSC质量和治疗潜力.
- 在临床应用之前,LRRC15可用于选择高质量的MSC用于细胞移植.
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