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相关概念视频

Nucleotide Excision Repair01:08

Nucleotide Excision Repair

Overview
Overview of DNA Repair02:25

Overview of DNA Repair

In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
Mutations01:35

Mutations

Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Other Unique Bacteria01:18

Other Unique Bacteria

Magnetic bacteria exhibit a directed movement called magnetotaxis, driven by structures called magnetosomes. These magnetosomes consist of chains of magnetic particles made of either magnetite (Fe₃O₄) or greigite (Fe₃S₄) and are organized in a linear conformation by a protein scaffold within invaginations of the cell membrane. The bacteria align along the north–south magnetic field lines, much like a compass needle. They are typically microaerophilic or anaerobic and are commonly found near the...

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从水溶液中的DNA核酸中产生超雷利散射.

Christian Jonin1, Maksymilian Dereniowski2, Estelle Salmon3

  • 1Laboratoire Charles Coulomb, University Montpellier, CNRS, Montpellier, France.

The Journal of chemical physics
|August 3, 2023
PubMed
概括

这项研究首次使用超雷利散射测量了DNA核酸的超极化性. 关氨酸和氨酸核酸分别显示出最高和最低的值,为其非线性光学特性提供了洞察力.

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科学领域:

  • 非线性光学是一种非线性光学.
  • 分子生物物理学的分子生物物理学.
  • 量子化学是一种量子化学.

背景情况:

  • 核酸是DNA的基本组成部分,对遗传信息存储至关重要.
  • 了解它们的电子和光学特性对于分子电子学和生物物理学至关重要.
  • 第一个超极化性 (β) 量化了分子对应用于电场的反应,表明非线性光学行为.

研究的目的:

  • 实验性地确定四个DNA核酸的第一个超极化性 (β):腺素,胆氨酸,细胞素和关氨酸.
  • 通过极化解析的测量来研究第一个超极化的张量对称性.
  • 将实验结果与使用密度函数理论 (DFT) 的理论计算进行比较.

主要方法:

  • 超雷利散射 (HRS) 技术在800纳米的非共振波长的水溶液中使用.
  • 进行极化解析的HRS测量以分析超极化度张量.
  • 使用PCM-B3LYP/6-31G+(d) 基础集进行密度函数理论 (DFT) 计算,包括溶剂效应.

主要成果:

  • 首次超极化值的范围从1.67 ± 0.15 × 10−30 esu (提米丁-5'-单酸盐) 到1.76 ± 0.16 × 10−30 esu (2'-瓜诺辛-5'-单酸盐) 之间.
  • 极化研究提供了关于第一个超极化的对称性和张量元素的见解.
  • 实验值与DFT理论预测有很好的一致性.

结论:

  • 该研究成功量化了DNA核酸的第一个超极化性,揭示了它们非线性光学反应的差异.
  • 实验和理论方法提供了关于分子超极化性的补充信息.
  • 这些发现有助于理解核酸光物理和分子材料的潜在应用.