相关实验视频

Updated: Jul 20, 2025

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

Published on: October 23, 2018

miR-21-5p促进与NASH相关的肝癌发生

Pedro M Rodrigues^1,2,3,4, Marta B Afonso¹, André L Simão¹

¹Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Lisbon, Portugal.

Liver international : official journal of the International Association for the Study of the Liver

|August 3, 2023

概括

微RNA-21-5p驱动非酒精性脂肪性肝病 (NAFLD) 进展为脂肪肝炎 (NASH) 和肝癌. 抑制这种微RNA可能为NASH提供一种新的治疗方法,并预防肝细胞癌 (HCC).

科学领域:

肝病学肝病学是一种肝病学.
分子生物学分子生物学
在瘤学瘤学.

背景情况:

非酒精性脂肪肝疾病 (NAFLD) 进展为脂肪肝炎 (NASH) 和肝细胞癌 (HCC) 的机制尚未完全理解.
需要对hsa-miRNA-21-5p在NASH相关肝癌发生中的作用进行研究.

研究的目的:

评估hsa-miRNA-21-5p在NAFLD向NASH和HCC的进展中的作用.
探索hsa-miRNA-21-5p作为NASH和HCC的潜在治疗点.

主要方法:

在NAFLD和HCC患者队列中评估肝脏hsa-miR-21-5p表达.
在肥胖的NAFLD患者中,与hsa-miR-21-5p相关的血清/肝脏代谢概况.
使用野生类型和Mir21淘汰赛小鼠食胆缺乏,氨基酸定义 (CDAA) 饮食以模拟NASH和NASH-HCC.

主要成果:

在肥胖患者中,hsa-miR-21-5p表达与NAFLD严重程度增加,并与脂毒性相关.
被CDAA养的野生型小鼠出现了NASH,纤维化和前瘤病变,而Mir21 KO小鼠显示肝损伤减少,并保护免受癌症发生.
在HCC患者中,hsa-miRNA-21-5p/PPARα通路被放松调节,与HCC标志物和不良预后相关.

结论:

hsa-miR-21-5p是NAFLD进展的关键驱动因素,从肥胖症到NASH和相关的致癌症.
抑制hsa-miR-21-5p促进了亲代谢的特征,这表明了预防NASH和HCC的治疗策略.

关键词:

在HCC中,HCC是HCC,HCC是HCC.纳什·纳什 (Nash Nash) 是一个名为纳什的城市.这是一种PPARα.这是一个hsa-miR-21-5pp.脂质新陈代谢脂质新陈代谢

更多相关视频

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

In Vitro Cultivation Techniques for Modeling Liver Organogenesis, Building Assembloids, and Designing Synthetic Tissues using Human Cell Lines

Published on: April 18, 2025

The Murine Choline-Deficient, Ethionine-Supplemented CDE Diet Model of Chronic Liver Injury

The Murine Choline-Deficient, Ethionine-Supplemented CDE Diet Model of Chronic Liver Injury

Published on: October 21, 2017

相关概念视频

Mutagenicity and Carcinogenicity

Mutagenicity and Carcinogenicity

Mutagenicity and carcinogenicity refer to the ability of drugs to cause genetic defects and induce cancer, respectively. The International Agency for Research on Cancer (IARC) classifies agents into four groups based on their carcinogenic potential. Group 1 agents are known human carcinogens; group 2A agents are probably carcinogenic to humans; group 3 agents lack data to support their role in carcinogenesis; and group 4 includes agents for which data support that they are not likely to be...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

Liver Regeneration

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...