相关实验视频
Updated: Jul 20, 2025
08:58
Optimized Analysis of In Vivo and In Vitro Hepatic Steatosis
Published on: March 11, 2017
16.0K
通过依赖m6A-YTHDF2的机制抑制NOVA2,METTL14可以逆转肝纤维化
Xiaoxue Hou1, Yuwen Li2, Jiali Song1
1Department of Infectious Disease, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Hepatology communications
|August 3, 2023
概括
该研究显示,降低METTL14水平通过减少N6-甲基氨酸 (m6A) 修饰,促进HSC激活和NOVA2mRNA降解来恶化肝纤维化. 恢复METTL14或NOVA2可以治疗肝纤维化.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 肝病学 肝病学是一种肝病学.
背景情况:
- N6-甲基氨酸 (m6A) 是一种关键的RNA修饰,参与各种生理和病理过程.
- 通过METTL14介导的m6A修饰在肝纤维化 (LF) 病原发生中的作用在很大程度上仍未被探索.
研究的目的:
- 为了调查METTL14诱导的m6A修饰在肝纤维化中的参与.
- 阐明METTL14影响肝星细胞 (HSC) 激活和LF进展的潜在分子机制.
主要方法:
- 在体外研究中使用具有METTL14倒置/过度表达的HSC细胞系来评估增殖,细胞周期和迁移.
- 使用MeRIP-seq和RNA-seq来识别METTL14下游目标.
- 在体内实验中使用AAV介导的METTL14敲击在LF的小鼠模型.
主要成果:
- 在LF患者的肝组织中,METTL14表达和m6A修饰水平降低.
- 在HSC中抑制METTL14促进了它们的激活,扩散和迁移,加剧了LF.
- METTL14耗尽减少了NOVA2mRNA的m6A修饰,导致其通过YTHDF2路径降解.
结论:
- 在肝纤维化中,METTL14作为瘤抑制剂,通过m6A修饰维持NOVA2的表达.
- 缺乏METTL14会加剧LF,而NOVA2则表现出抗纤维的作用.
- 准METTL14-m6A-NOVA2轴为肝纤维化提供了一个潜在的治疗策略.
更多相关视频
10:42Development of an Ethanol-induced Fibrotic Liver Model in Zebrafish to Study Progenitor Cell-mediated Hepatocyte Regeneration
Published on: May 13, 2016
9.2K
08:04Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method
Published on: October 23, 2018
19.0K
相关概念视频
01:24Liver Regeneration
3.3K
The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
3.3K
01:16TGF - β Signaling Pathway
7.4K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.4K