在Proteus mirabilis的临床分离物中,病毒性因素和多药耐药性之间的相关性研究
Mai Elhoshi1, Eglal El-Sherbiny1, Amel Elsheredy1
1Department of Microbiology, Medical Research Institute, Alexandria University, Alexandria, Egypt.
概括
由于耐药性和毒性因素,Proteus mirabilis感染很难治疗. 这项研究没有发现临床分离物中多药耐药性 (MDR) 和毒性之间的直接联系,尽管生物膜形成很常见.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 蛋白质奇迹菌感染由于高毒性和抗菌素耐药性而带来治疗挑战.
- 多药性耐药性 (MDR) 和广泛的耐药性 (XDR) 复杂化了P.mirabilis的感染控制.
- 了解毒性和耐药性之间的关系对于有效的治疗策略至关重要.
研究的目的:
- 调查临床P.奇迹菌分离物中毒性决定因素和多药性耐药性之间的相关性.
- 分析特定毒性因子和抗菌素耐药性模式的流行情况.
- 确定生物膜形成与抗菌素耐药性之间的关联.
主要方法:
- 从亚历山大 (2019年12月 - 2021年6月) 收集了100个临床P. mirabilis分离物.
- 使用Kirby Bauer方法评估抗微生物敏感性.
- 对特定基因 (zapA, flaA, ureC, mrpA, atfA, ucaA, hpmA, luxS) 进行表型和通过PCR检测的毒性因子 (蜂群,尿酶,蛋白酶,血清素,生物膜).
主要成果:
- 在34%和5%的分离物中观察到MDR和XDR.
- 所有分离物体都表现出运动性,蜂群,尿酶和蛋白质酶的产生;90%产生了血清素,73%形成了生物膜.
- 虽然病毒性基因的普遍性很高,但MDR和整体病毒性没有显示出统计相关性. 生物膜的产生与尿管隔离物有关,在MDR/XDR菌株中很常见.
结论:
- 从导管化尿样本中分离的P. mirabilis表明与生物膜形成有显著的关联.
- 在这项研究中,多药耐药性和毒性因素之间没有发现统计相关性.
- 尽管非MDR分离物中毒性因子丰富度高,但与MDR分离物相比没有观察到显著差异.
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