系统分析确定UBE2C是上皮癌的新瘤基因标
Renlun Huang1,2, Lang Guo1,3, Chiwei Chen1,2
1The Research Center of Integrative Cancer Medicine, Discipline of Integrated Chinese and Western Medicine, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
PloS one
|August 3, 2023
概括
乌比奎丁结合酶2C (UBE2C) 驱动上皮层癌 (ACC) 的瘤进展. 高UBE2C表达与预后不佳相关,促进细胞循环和EMT,使其成为潜在的治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 乌比基结合酶2C (UBE2C) 与瘤进展有关.
- 在上皮癌 (ACC) 中UBE2C的特定作用和机制尚不清楚.
- 研究UBE2C的瘤效应可以澄清其在ACC的预后价值.
研究的目的:
- 系统地调查UBE2C在上皮癌 (ACC) 中的瘤发生效应和预后价值.
- 探索UBE2C影响ACC进展的潜在机制,包括细胞周期和上皮-介质酶过渡 (EMT).
主要方法:
- 通过ACLBI网络工具对ACC的癌症基因组图谱 (TCGA) RNA-seq数据进行了利用生物信息学分析.
- 通过交叉DFS相关基因,OS相关基因,ACC中高度表达的基因和差异表达基因 (DEGs) 来识别候选基因.
- 进行了体外实验,涉及UBE2C在ACC细胞中的淘汰,以评估增殖,迁移,入侵,EMT和细胞周期进展.
主要成果:
- UBE2C被确定为ACC瘤和正常组织之间最重要的DEG,高表达与预后不佳有关.
- UBE2C表达与细胞循环调节剂 (CDC20,CDK1,CCNA2) 有正相关,表明细胞循环进展过度活跃.
- 在实验室中,UBE2C Knockdown显著抑制了ACC细胞的增殖,迁移,入侵,EMT和细胞周期进展.
- 全癌症分析显示,UBE2C是多种瘤类型的瘤基因.
结论:
- 高UBE2C表达与ACC患者的预后不佳密切相关.
- UBE2C通过增强细胞周期进展和EMT来促进ACC进展.
- UBE2C是新型ACC疗法的一个有前途的分子标.
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