Search research articles

相关概念视频

Tagging and Fusion Proteins

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Christina Kim: functional and biochemical mapping of neural circuits.

Nature reviews. Neurology·2026

Same author

D3 dopamine receptors implicate a striatal neuronal subtype in the aversive effects of the antipsychotic drug quetiapine.

bioRxiv : the preprint server for biology·2026

Same author

Deconstruction of a memory engram reveals distinct ensembles recruited at learning.

Nature neuroscience·2026

Same author

High-speed neural imaging with multiplexed miniaturized two-photon microscopy.

Cell reports methods·2025

Same author

The psychoplastogen tabernanthalog induces neuroplasticity without proximate immediate early gene activation.

Nature neuroscience·2025

Same author

Deconstruction of a Memory Engram Reveals Distinct Ensembles Recruited at Learning.

Research square·2025

Same journal

Erratum for the Research Article "Detecting supramolecular organic nanoparticles during heat wave".

Science (New York, N.Y.)·2026

Same journal

Local signals, systemic decline.

Science (New York, N.Y.)·2026

Same journal

The mechanics of liver regeneration.

Science (New York, N.Y.)·2026

Same journal

Computing in a memory with physics.

Science (New York, N.Y.)·2026

Same journal

Retraction.

Science (New York, N.Y.)·2026

Same journal

Making time.

Science (New York, N.Y.)·2026

查看所有相关文章

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

Search research articles

相关实验视频

Updated: May 11, 2026

DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices

DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices

Published on: July 6, 2010

用光标记神经元

Christina K Kim¹

¹Center for Neuroscience and Department of Neurology, University of California, Davis, Davis, CA, USA.

Science (New York, N.Y.)

|August 3, 2023

概括

此摘要是机器生成的。

研究人员开发了基于细胞活动的新型分子电路来控制光遗传学. 这种进步使得神经刺激更加精确和响应,

更多相关视频

Using Affordable LED Arrays for Photo-Stimulation of Neurons

Using Affordable LED Arrays for Photo-Stimulation of Neurons

Published on: November 15, 2011

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Published on: December 12, 2012

相关实验视频

Last Updated: May 11, 2026

DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices

DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices

Published on: July 6, 2010

Using Affordable LED Arrays for Photo-Stimulation of Neurons

Using Affordable LED Arrays for Photo-Stimulation of Neurons

Published on: November 15, 2011

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Targeted Labeling of Neurons in a Specific Functional Micro-domain of the Neocortex by Combining Intrinsic Signal and Two-photon Imaging

Published on: December 12, 2012

科学领域:

神经科学
分子生物学
生物技术

背景情况:

视觉遗传学可以通过光来精确控制神经元活动.
目前的光遗传方法往往缺乏对细胞状态的实时响应.
发展活动依赖控制对于理解复杂的神经回路至关重要.

研究的目的:

工程分子电路将细胞活动与光遗传控制联系起来.
创建一个神经元功能动态和响应调节的系统.
为了研究神经电路动力学的工具.

主要方法:

合成基因电路的设计和构造.
活动传感器与光遗传驱动器的集成.
用细胞和/或体内模型进行验证.

主要成果:

已经证明了光遗传工具的成功活动依赖激活.
通过内源活动来精确控制神经元发射.
描述了分子电路的性能和特异性.

结论:

活动指导的光遗传学为神经电路询问提供了一种强大的新方法.
这种方法可以对神经活动进行更复杂和生物相关的操纵.
未来的应用包括研究神经可塑性和治疗神经疾病.