YY:维护Candida肠道共生的一种帕尼特细胞抗菌
Joseph F Pierre1,2, Brian M Peters3, Diana La Torre1
1Department of Medicine, University of Chicago, Chicago, IL, USA.
概括
由肠道Paneth细胞产生的YY (PYY1-36) 作为一种抗真菌抗菌 (AMP) 的功能. 它选择性地向Candida albicans,破坏膜,促进肠道真菌平衡.
科学领域:
- 微生物学
- 胃肠病学
- 免疫学
背景情况:
- 哺乳动物的肠道调节食欲,分泌物,机动性和微生物群.
- 肠表皮中的帕尼特细胞 (PCs) 分泌各种.
- 特定Y (PYY) 异型在肠道防御中的作用尚未完全理解.
研究的目的:
- 研究由肠道帕内特细胞 (PCs) 生产的YY (PYY1-36) 的抗菌特性.
- 确定PC-PYY抗菌活性的具体目标和机制.
- 阐明PC-PYY在维持肠道真菌共生中的作用.
主要方法:
- 作为抗菌 (AMP) 的PC-PYY的特征.
- 评估PC-PYY的抗菌和抗真菌活性.
- 分析PC-PYY与真菌菌的相互作用,包括膜破坏和转录变化.
- 评估PC-PYY对Candida albicans形态的影响 (菌与酵母菌).
主要成果:
- YY (PYY1-36),但不是PYY3-36,由肠道帕内特细胞 (PC) 表达,并作为抗菌 (AMP) 起作用.
- PC- PYY对毒性Candida albicans hyphae具有选择性的抗真菌活性,但对酵母形式没有.
- 一个电离子分子PC-PYY破坏了真菌的阳离子表面,导致膜损伤和转录重编程.
- 这种重新编程有利于酵母表型,有助于维持肠道真菌的共生.
结论:
- 肠上皮细胞分泌YY (PYY1-36) 作为一种抗真菌抗菌 (AMP).
- 通过PC-PYY选择性地向和破坏Candida albicans hyphae,有助于调节肠道真菌种群.
- PC-PYY在哺乳动物肠道内维持真菌初生的平衡中发挥作用.
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