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非洲猪瘟病毒pF778R通过阻碍STAT1核转位减弱I型干扰素反应

Qichao Chen¹, Liang Li¹, Lixinjie Liu¹

¹National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.

|August 3, 2023

概括

非洲猪瘟病毒 (ASFV) 使用pF778R蛋白质逃避宿主免疫力. 这种蛋白质通过阻断STAT1进入核的运动来抑制I型干扰素反应,这对抗病毒防御至关重要.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

非洲猪瘟病毒 (ASFV) 在没有疫苗的情况下导致了毁灭性的猪疫情.
ASFV使用许多蛋白质来抑制基于宿主干扰素 (IFN) 的免疫力.
了解ASFV免疫逃避是制定控制策略的关键.

研究的目的:

研究ASFV蛋白质在逃避I型IFN反应中的作用.
为了确定新的ASFV机制来对抗天生的免疫力.

主要方法:

评估了ASFV蛋白质pF778R对I型IFN信号传输的影响.
分析了IFN刺激反应元件 (ISRE) 促进者的活动.
量化的干扰素刺激基因表达 (ISG).
研究了STAT1的酸化,二聚化和核转位.
在JAK/STAT路径内检查的相互作用.

主要成果:

ASFV pF778R显著抑制I型IFN反应,包括ISRE激活和ISG上调.
pF778R可以防止STAT1的核转移,而不会影响其酸化或二聚化.
该蛋白质通过阻碍STAT1核积累来阻碍JAK/STAT通路中介的IFN信号传递.
pF778R不会改变关键JAK/STAT通路组件的蛋白质水平,也不会与它们直接相互作用.

结论:

ASFV蛋白 pF778R 是一种新型的IFN反应的新型对手.
pF778R通过抑制STAT1核积累来规避先天免疫,这是ASFV独特的免疫规避策略.
这一发现提供了关于ASFV病原体和潜在治疗点的见解.

关键词:

非洲猪瘟病毒病毒免疫逃逸是一种免疫逃避.STAT1 核转位转位类型I IFN信号传输 pF778RR 在线播放

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