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雄性化基因控制鱼,Cydia pomonella的男性性别决定
Kristýna Pospíšilová1, Arjen E Van't Hof2, Atsuo Yoshido2
1Biology Centre of the Czech Academy of Sciences, Institute of Entomology, 370 05, České Budějovice, Czech Republic; Faculty of Science, University of South Bohemia, 370 05, České Budějovice, Czech Republic.
Insect biochemistry and molecular biology
|August 3, 2023
概括
雄性化基因 (Masc) 控制鱼的雄性发育. 在ZZ胚胎中击败CpMasc将性别决定转移到女性,证明其在男性发育中的关键作用.
科学领域:
- * 分子生物学 * 分子生物学
- * 昆虫学 昆虫学
- * 遗传学 在遗传学方面
背景情况:
- * 在雌性异构性 (WZ/ZZ) 的Lepidoptera (和蝶) 中,除了在Bombyx mori.外,性决定在很大程度上是未知的.
- *男性化基因 (Masc) 对于B. mori的男性发育和剂量补偿至关重要.
- * Masc 的男性化功能在几种物种中得到保护.
研究的目的:
- * 为了研究Masc基因在鱼,Cydia pomonella的性别决定中的作用.
- * 分析C. pomonella Masc ortholog (CpMasc) 的基因结构和拼接变体.
- * 确定C. pomonella中CpMasc的性别特异性表达和功能.
主要方法:
- * CpMasc. 的基因结构分析.
- *使用转录组数据和RT-PCR识别和选CpMasc拼接变异.
- *定量PCR (qPCR) 分析CPMasc变异的性别偏差表达.
- *RNA干扰 (RNAi) 在胚胎发生过程中降低CpMasc表达.
- *对下游效应基因的分析,C. pomonella双性 (Cpdsx),拼接模式.
主要成果:
- * CpMasc与B. mori Masc具有结构上的相似性,但表现出14种拼接变体.
- *确定了三个男性特异性的CpMasc拼接变体,早期男性胚胎的性别偏差表达达到峰值.
- *在ZZ胚胎中,RNAi介导的CpMasc的淘汰导致了Cpdsx的转向女性特异性拼接.
- * Knockdown导致后代的性别比例偏向于女性.
- * 在未受精和成熟的卵子中检测到cpMasc转录,表明母亲提供.
结论:
- * CpMasc 在C. pomonella的性别决定级联中起到男性化基因的作用.
- *已识别的拼接变体及其性别特异性表达对男性发育至关重要.
- * CpMasc mRNA的母体供应是Lepidoptera性别确定的一个独特特征.
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