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Updated: Jul 20, 2025

Targeted DNA Methylation Analysis by Next-generation Sequencing

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Published on: February 24, 2015

基于滴滴的双硫酸盐测序用于单细胞DNA甲基组的高通量分析

Qiang Zhang¹, Sai Ma^2,3, Zhengzhi Liu²

¹Department of Chemical Engineering, Virginia Tech, Blacksburg, VA, 24061, USA.

Nature communications

|August 3, 2023

概括

一种新的基于滴滴的微流体技术,Drop-BS,可以实现可扩展的单细胞DNA甲基组概况. 这种方法有效地处理多达1万个细胞,揭示复杂组织中细胞类型的异质性.

科学领域:

表观遗传学和基因组学
一个单细胞分析.
微流体学微流体学

背景情况:

DNA甲基组对于调节基因活动和细胞命运至关重要.
单细胞DNA甲基组研究为细胞子集识别提供了高分辨率.
目前的方法缺乏可扩展性来分析大量单个细胞.

研究的目的:

开发一种可扩展的基于滴滴的微流体技术,用于单细胞DNA甲基组分析.
为了实现单细胞二硫酸盐测序库的高通量构建.
克服现有的基于管或井板的平台的局限性.

主要方法:

开发一种以滴滴为基础的微流体系统,命名为Drop-BS.
在图书馆准备中使用超高吞吐量滴滴微流体.
适用于分析混合细胞系,小鼠和人类大脑组织.

主要成果:

Drop-BS成功地准备了单细胞二硫酸盐测序库.
该技术可以在2天内处理多达1万个单细胞.
对各种组织的分析揭示了细胞类型的异质性.

结论:

Drop-BS为单细胞DNA甲基组分析提供了可扩展和高效的解决方案.
这项技术有助于对大型细胞群体进行检查.
在单细胞分辨率下,可以更深入地了解表观基因组异质性.

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Optimized Analysis of DNA Methylation and Gene Expression from Small, Anatomically-defined Areas of the Brain

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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...