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无标签测量抗微生物与脂质囊泡和纳米磁盘的相互作用,使用微观温度测量
Philip Rainsford1, Fredrik G Rylandsholm1, Martin Jakubec1
1Department of Chemistry, Faculty of Science and Technology, UiT the Arctic University of Norway, 9019, Tromsø, Norway.
Scientific reports
|August 3, 2023
概括
微尺度热泳 (MST) 提供了一种无标签的方法来测量抗生素与膜的结合亲和力 (KD). 这种技术有助于发现新的抗生素,通过有效评估脂相互作用,而无需昂贵的标签.
科学领域:
- 生物化学 生物化学
- 微生物学 微生物学
- 分析化学 分析化学
背景情况:
- 抗菌素耐药性需要发现新的抗生素类别.
- 抗生素的有效性往往取决于与细菌膜的相互作用.
- 量化膜结合亲和力 (KD) 和分离 (KP) 对于抗生素开发至关重要.
研究的目的:
- 建立微尺度热泳 (MST) 作为确定KD的无标签方法.
- 评估内在托芬光对KD测量的实用性.
- 评估MST用于研究与抗生素发现相关的脂相互作用.
主要方法:
- 使用微尺度热泳 (MST) 具有内在的基基光,用于无标签的KD测量.
- 应用MST来测量循环AMP与大单囊 (LUV) 和SMA/SMA-QA纳米光盘的结合.
- 经验证的MST结果与表面等离子体共振 (SPR) 和最小抑制度 (MIC) 数据相比.
主要成果:
- 通过MST和内在光成功证明了无标签的KD确定.
- 获得了与SPR测量良好相关的KD值.
- 测量KD和抗菌活性 (MIC) 与*S. aureus*和*E. coli*之间显示出广泛的相关性.
结论:
- MST是一种可行的,无标签的技术,用于测量脂结合亲缘关系.
- 该方法为抗菌候选药物的初始查提供了一种快速和成本效益的方法.
- 在资源密集型分析之前,MST对于绘制样本条件有价值.
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