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抑制EGFR抑制了MCF7细胞过度表达EGFR的球体形成

D D Novak¹, O S Troitskaya¹, A A Nushtaeva¹

¹Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090 Russian Federation.

|August 4, 2023

概括

表皮生长因子受体 (EGFR) 抑制通过影响N-cadherin水平来减少乳腺癌球状体的形成. 这表明EGFR对于球体组合至关重要,为侵略性瘤提供了一个模型.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

皮表皮生长因子受体 (EGFR) 是一种驱动瘤生长的氨酸激酶.
EGFR抑制剂对于抗癌疗法至关重要.
在MCF7细胞 (MCF7-EGFR) 中的EGFR表达促进了3D球状体的生长.

研究的目的:

研究EGFR抑制对球体组装和破坏的影响.
为了比较抗EGFR siRNA, cetuximab 和 AG1478 对MCF7-EGFR细胞的疗效.
为了阐明N-cadherin在EGFR依赖球形形成中的作用.

主要方法:

比较 cetuximab 和 AG1478 对 MCF7-EGFR 和父母 MCF7 细胞的细胞毒性作用.
用siRNA.RNA抑制EGFRmRNA后评估球体的形成.
评估 cetuximab 和 AG1478 对球状体形成和 N-cadherin 水平的影响.

主要成果:

MCF7-EGFR细胞对 cetuximab 和 AG1478.8 的敏感性提高了 2.5 倍.
抑制EGFRmRNA减少了球状体的形成;先前存在的球状体不受影响.
塞图西马布和AG1478抑制了分离细胞中的球状体的形成.
在球状转变期间,N-cadherin水平增加,在EGFR抑制时降低.

结论:

对于球状体的形成而不是破坏而言,EGFR表达是至关重要的.
N-cadherin参与了依赖EGFR的球体组合.
MCF7-EGFR球状体作为激素阳性乳腺瘤的模型.

关键词:

3D细胞培养的3D细胞培养 AG147878 AG1478 AG1478 AG1478 AG1478 AG1478 AG1478 AG1478 AG1478 AG1478 AG1478 AG1478 AG1478 AG1478 AG1478 AG1478 AG1478 AG1478 AG1478 欧洲农业基金会 (EGFR) 是一个基金.MCF7 MCF7 MCF7 MCF7 MCF7 MCF7 MCF7 MCF7 MCF7 这就是 cetuximab 的意思.siRNA 是一个RNA.球形状的球形状是指球形状的球形状.

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