超明亮的双光子可激发的红色发射化探头,用于快速且无需洗地在活细胞中进行生物对等标签
Marie Auvray1,2, Delphine Naud-Martin1,2, Gaëlle Fontaine1,2
1CNRS UMR9187, Inserm U1196, Chemistry and Modeling for the Biology of Cancer Institut Curie, Université PSL 91400 Orsay France marie.auvray1@gmail.com florence.mahuteau@curie.fr.
Chemical science
|August 4, 2023
概括
研究人员开发了新的化探针,用于生物对等化学. 这些探测器可以通过两光子激发进行深层组织成像,提供更好的信号噪声比率和减少在体内跟踪分子的光损伤.
科学领域:
- 生物对等化学的化学.
- 光成像成像的使用方法
- 化学生物学是化学生物学.
背景情况:
- 化生物对等反应对于生命系统中跟踪分子至关重要.
- 双光子激发增强成像深度和信号对噪声比,同时最大限度地减少光损伤.
- 存在对高效的二光子可激发化探头的需求.
研究的目的:
- 设计和合成具有双光子激发能力的新型化探针.
- 评估这些探针在生物对等反应中的动力和效性能.
- 为了证明它们在活细胞和体内成像应用中的实用性.
主要方法:
- 合成含有两光子可激发光体和氨酸灭部分的化探针.
- 使用双环[6.1.0]no-4-yn-9ylmethanol (BCN) 的探针反应的动力分析.
- 在活细胞中,在一光和两光子激发下测量光强度.
- 使用时间依赖密度函数理论 (TDDFT) 进行理论计算.
主要成果:
- 探测器表现出与NCB的良好至令人印象深刻的动力速率常数 (高达1.1 × 10^3 M^-1 s^-1).
- 发射红色的探针显示了中等到高的光激活比率 (在活细胞中高达13.8倍).
- 点击的 adduct 的高两光子亮度 (>300 GM) 便于使用低激光功率进行体内成像.
结论:
- 开发的化探针是生物对等化学和活细胞成像的有效工具.
- TDDFT成功地使探头性能合理化,帮助合理设计.
- 这些探头能够进行深层组织和体内成像,减少光损伤和高信号噪声比.
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