控制人类cDC2分化和单细胞重编程的微环境和细胞内在因素
Magdalena Lang1, Corinna Krump1, Anastasia Meshcheryakova2
1Division of Immunology, Otto Loewi Research Center, Medical University of Graz, Graz, Austria.
Frontiers in immunology
|August 4, 2023
概括
皮肤中的炎症信号,如BMP7,驱动血液树突细胞2s (cDC2s) 分化为特定的组织居住子集,包括Axl + cDC2s和RelB + cDC2s.
科学领域:
- 免疫学 免疫学 免疫学
- 皮肤病学 皮肤病学
- 细胞生物学 细胞生物学
背景情况:
- 循环血液中的cDC2s在外周组织中很丰富.
- 控制组织中cDC2分化的因素,特别是在炎症期间,尚不清楚.
研究的目的:
- 为了研究在牛皮皮肤中控制cDC2分化的因素.
- 了解炎症信号如何影响cDC2可塑性.
主要方法:
- 对疹cDC2s的分析,用于细胞酶受体Axl和信号通路.
- 在实验室中,使用BMP7和TGF-β1.1.从血液中诱导Axl+cDC2s.
- 使用BMP7和CD34+造血原生细胞建模cDC2生成和分化.
- 调查p38MAPK在cDC2子集生成中的作用.
- 对cDC2s中的RelB+cDC2s和KLF4表达的分析.
主要成果:
- 牛皮的cDC2s表达了Axl,并显示了BMP和p38MAPK信号.
- 在体外,BMP7和TGF-β1从血液中的cDC2s中诱导Axl+cDC2.
- p38MAPK驱动Langerhans细胞上的Axl+ cDC2s,并促进RelB+ cDC2s.
- BMP7可以模拟cDC2和Langerhans细胞与原始细胞的分化.
- cDC2s可以获得单细胞衍生的DC和巨细胞特征,包括KLF4表达.
结论:
- 牛皮皮肤中的炎症信号指示血液cDC2s分化为各种组织居民细胞子集.
- BMP7和p38MAPK信号是cDC2分化和可塑性的关键调节者.
- cDC2s表现出显著的可塑性,在炎症条件下获得其他骨髓系的表型.
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