纤维细胞衍生矩阵模拟脱塑性质,并形成癌症进展中的预后特征
Maria Rafaeva1, Adina R D Jensen1, Edward R Horton1
1Biotech Research and Innovation Centre, University of Copenhagen, Copenhagen, Denmark.
Frontiers in immunology
|August 4, 2023
概括
癌症的进展与瘤微环境的变化有关,特别是细胞外基质 (ECM). 这项研究确定了与癌症进展和患者存活率相关的36种蛋白质ECM签名.
科学领域:
- 在瘤学瘤学.
- 生物化学 生物化学
- 癌症生物学 癌症生物学
背景情况:
- 脱质反应的特征是变化的肌体细胞外基质 (ECM),在癌症进展中至关重要,特别是在乳腺癌和胰腺癌中.
- 纤维细胞衍生矩阵 (FDM) 是瘤微环境的关键组成部分,并在癌细胞行为中发挥重要作用.
研究的目的:
- 为了研究癌症相关性肌层与正常肌层相比,在癌症相关性肌层内ECM特征的改变.
- 确定这些ECM变化对癌细胞生长的影响.
- 为了识别与癌症进展和患者预后相关的特定ECM签名.
主要方法:
- 从正常和与癌症相关的纤维细胞中利用纤维细胞衍生矩阵 (FDM).
- 对FDM进行了组合,结构和机械分析.
- 导出并验证了36-ECM蛋白签名与胰腺管腺癌 (PDAC) 进展以及患者的转录组数据.
主要成果:
- 与正常的FDM相比,癌细胞在与癌症相关的FDM上表现出增长.
- 与癌症相关的FDM显示了核心ECM蛋白的丰富性增加,增强了刚性和密度.
- 一个36-ECM蛋白签名来自FDM组成变化与PDAC进展和多种癌症类型的患者存活相关.
结论:
- 来自纤维细胞的矩阵是研究与癌症相关的脱形成的相关模型.
- 鉴定到的36-ECM蛋白签名具有预后价值,可以指导进一步的机理学研究.
- 对于癌症的进展和治疗策略来说,了解脱质变化的ECM变化至关重要.
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