APLNR标志着由人类诱导的多能干干细胞衍生出来的心脏原始体
Yin-Yu Lam1, Chun-Ho Chan1, Lin Geng2
1Department of Paediatrics and Adolescent Medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, China.
Heliyon
|August 4, 2023
概括
研究人员将APLNR确定为一种新型的表面标记物,用于从人类诱导的多能干细胞 (hiPSCs) 中分离心脏原始细胞. 这种方法提高了产生心肌细胞用于心脏研究和再生的一致性.
科学领域:
- 干细胞生物学 干细胞生物学
- 发展生物学 发展生物学
- 心血管研究研究心血管研究
背景情况:
- 从人类诱导的多能干细胞 (hiPSCs) 产生心肌细胞显示出不同细胞系和批次的可变效率.
- 试管心脏分化中的不一致性阻碍了疾病建模和再生医学中的可靠应用.
研究的目的:
- 为了确定可靠的标记物,在hiPSC分化过程中隔离心脏原生细胞.
- 开发一个强大的平台,以提高一致性,从多种hiPSC系产生心肌细胞.
主要方法:
- 在心脏分化过程中,在hiPSC上使用单细胞转录组学来绘制细胞系谱.
- APLNR被确定为心脏原始体的表面标记物,然后使用免疫磁性分离来隔离.
- 对隔离的APLNR+心脏原始体进行了转录组分析,并与体内胚胎数据集进行了比较.
主要成果:
- 单细胞转录组学在hiPSC分化过程中揭示了不同的心脏和心脏外血统.
- 鉴定出APLNR+细胞是体外心脏前体的特定种群.
- 隔离的APLNR+原体的分化结果主要是心肌细胞和心脏介质细胞,具有暂时的原体标记表达.
- 在人类和小鼠胚胎中,APLNR表达在早期心脏原始体中得到验证.
结论:
- APLNR作为一种可靠的表面标记物,可以在未经遗传修饰的情况下从多个hiPSC线路中分离出体外心脏原始体.
- 这个平台提供了一种一致的方法来产生心肌细胞和心脏介质细胞,适用于心脏发育研究,疾病建模和再生医学.
- 这些发现为从干细胞中产生更可预测和更可扩展的心肌细胞的基础.
相关概念视频
01:13Induced Pluripotent Stem Cells
24.2K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
24.2K
01:22iPS Cell Differentiation
2.7K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.7K
01:17Stem Cell Culture
5.2K
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
5.2K