早期的原子弹辐射加速了免疫衰老,并提升了与免疫相关的细胞内活性氧物种
Tomonori Hayashi1,2, Naohiro Kato3, Keiko Furudoi2
1Department of Molecular Biosciences, Radiation Effects Research Foundation, Hiroshima, Japan.
Aging cell
|August 4, 2023
概括
辐射暴露和衰老减少了天真T细胞,增加了氧化应激. 这项研究将较低的天真CD4+T细胞数量与CD8+T细胞中较高的活性氧物种 (ROS) 联系起来,这表明免疫功能受损.
科学领域:
- 免疫学 免疫学 免疫学
- 辐射生物学 辐射生物学
- 衰老研究研究 衰老研究
背景情况:
- 反应性氧物种 (ROS) 对免疫反应至关重要,但过度水平会促进炎症和疾病.
- 辐射加速免疫衰老,但确切的机制仍然难以捉摸.
- 了解ROS在辐射诱导的免疫衰老中的作用至关重要.
研究的目的:
- 调查衰老和辐射暴露对T细胞子集细胞内ROS水平的影响.
- 为了检查T细胞子集百分比和辐射暴露后细胞内ROS水平之间的关系.
- 阐明ROS在辐射诱导的免疫衰老中的作用.
主要方法:
- 分析了来自3752名原子弹幸存者的10,023个样本 (2008-2016).
- 流细胞计用于测量细胞内ROS (H2O2,O2•−) 和T细胞子集百分比 (原始,记忆CD4+,CD8+).
- 用光标记的抗体和试剂 (carboxy-DCFDA,乙) 用于细胞和ROS检测.
主要成果:
- 原始CD4+和CD8+T细胞的百分比随着年龄和辐射剂量的增加而下降.
- 细胞内超氧化物 (O2•−) 水平在中央和效应记忆CD8+T细胞中升高.
- 在CD8+T细胞中增加的O2•−与较低的原始CD4+T细胞百分比相关,特别是在低辐射剂量下.
结论:
- 辐射暴露通过减少原始的CD4+T细胞,有助于免疫衰老.
- 这种原始T细胞的减少可能会损害免疫功能,导致ROS的产生增加.
- 在CD8+T细胞中细胞内ROS升高表明辐射暴露后氧化应激增加.
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