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机械适应在微型表皮的连续解释,通过体外实验得到了信息

Bernard L Cook¹, Patrick W Alford¹

¹Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN 55455, USA.

Integrative biology : quantitative biosciences from nano to macro

|August 4, 2023

概括

表皮组织通过机械适应适应机械应力,通过细胞骨变化和增殖调节应力. 这一过程对于组织的修复和发育至关重要.

科学领域:

细胞生物学细胞生物学
生物物理学的生物物理.
组织工程是组织工程.

背景情况:

表皮组织表现出机械适应,根据机械力量调整形状和功能.
这种适应往往涉及到反动的力量反对应用的变化,这表明机械的平衡.
表皮机械稳态对于胚胎形态发生,伤口愈合和成人组织维护至关重要.

研究的目的:

通过使用细胞微轴向拉伸 (CμBS) 来研究不同形状的微模式上皮组织中的机械适应.
了解表皮组织如何调节机械恒温,以应对受控的机械干扰.

主要方法:

利用CμBS平台,将30%的伸展应用于微模式的Madin-Darby犬细胞组织,持续24小时.
分析了组织压力,actin细胞骨组织和细胞增殖的变化.
开发并使用基于连续性的模型,包括压力诱导的actin重塑和扩散.

主要成果:

伸展后组织应力立即增加,并在24小时内逐渐恢复到伸展前的水平.
异性质组织显示出异性质应激模式,与actin细胞骨对齐增加后拉伸和随着时间的推移重组.
在未拉伸的组织中,增殖诱导的细胞封闭和厚度变化导致压力降低.

结论:

表皮机械适应取决于细胞结构和增殖.
一个基于连续性的模型有效地捕捉实验行为,这表明它适用于研究上皮质机械平衡.
这种结合实验和建模的方法为测量机械适应现象提供了一个系统的方法.

关键词:

马丁·达比狗脏狗脏连续性建模连续性建模它的表皮质 (epithelium) 是一个表皮质.机械适应机械适应拉力力显微镜拉力力显微镜

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