基因组变异与表观遗传修饰相结合,重新连接大米中的开放色素
Mengqi Li1, Yilong Feng1, Qi Han1
1State Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, CIC-MCP, Nanjing Agricultural University, No.1 Weigang, Nanjing, Jiangsu 210095, China.
Plant physiology
|August 4, 2023
概括
米 (Oryza sativa) 的序列和结构变化影响农学特征. 可转移元素通过染色体开放驱动基因变化,为作物改进提供目标.
科学领域:
- 植物基因组学 植物基因组学
- 分子遗传学 分子遗传学
- 农作物科学 农作物科学
背景情况:
- 细胞调节元件 (CREs) 调节基因转录,对作物化至关重要.
- 了解大米 (Oryza sativa) 亚种印加和日本的序列和结构变异 (SVs) 对于解释特征差异至关重要.
研究的目的:
- 调查全球序列和SVs在印加米和日本米之间的多层次变化中的作用.
- 阐明CREs的变化如何导致农学特征分歧.
主要方法:
- 整合MNase过敏测序 (MH-seq),RNA测序 (RNA-seq),ChIP-seq和双硫酸盐测序 (BS-seq) 的多原子研究.
- 全基因组关联研究 (GWAS) 与MNase过敏部位 (MHS) 数据相结合.
- 对可转移元素 (TE) 插入的分析及其对染色质可访问性的影响.
主要成果:
- 不同的MHS显示了日本米 (Nipponbare) 和印加米 (93-11) 品种之间明显的基因组序列特征.
- 携带CREs的MHS内部的序列变化与农学特征差异有关.
- 来自SV的MHS,特别是来自非常见的TE,与具有独特功能的基因有关,通过染色质开放性变化进行介导.
结论:
- 基因组SV和CREs的序列变化显著影响大米的农学特征.
- 可转移的元素在基因新或亚功能化中通过染色质修饰发挥关键作用.
- 这项研究为米分子育种中的基因组编辑提供了有价值的目标,以增强可取的农学特性.
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