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通过基于纳米的实时免疫PCR对肺外结核病例中的真菌细菌蛋白质进行量化

Bhawna Dahiya¹, Tulika Prasad², Anam Rais²

¹Centre for Biotechnology, Maharshi Dayanand University, Rohtak, 124001, Haryana, India.

Future microbiology

|August 4, 2023

概括

一种新的基于纳米的测定方法可以准确检测肺外结核病 (EPTB) 生物标志物. 这种快速诊断测试显示出高灵敏度和特异性,为EPTB诊断提供了一个有前途的工具.

科学领域:

纳米技术纳米技术
生物技术是生物技术.
传染性疾病传染性疾病

背景情况:

肺外结核病 (EPTB) 的诊断存在重大挑战.
迫切需要EPTB的快速和可靠的诊断方法.

研究的目的:

为了评估一种新的基于纳米的测定方法,SYBR绿色磁珠合黄金纳米粒子实时免疫聚合酶链反应 (MB-AuNP-RT-I-PCR),用于量化Mycobacterium结核病蛋白质.
在临床怀疑EPTB患者中评估MB-AuNP-RT-I-PCR的诊断准确性.

主要方法:

开发和应用MB-AuNP-RT-I-PCR测定方法,用于定量检测MPT-64+CFP-10蛋白质.
将MB-AuNP-RT-I-PCR与磁铁ELISA进行蛋白质定量和诊断性能的比较.

主要成果:

MB-AuNP-RT-I-PCR量化了MPT-64+CFP-10在一个广泛的范围 (270 fg/ml-9.9 ng/ml),超过了磁铁ELISA的狭窄范围 (1.8-10 ng/ml).
该试验在区分EPTB病例与非结核病对照时实现了高灵敏度 (88.2%) 和特异性 (100%).
与MB-AuNP-RT-I-PCR和磁铁ELISA相比,MB-AuNP-RT-I-PCR显示出显著更高的敏感性.

结论:

在MB-AuNP-RT-I-PCR测定表现出良好的诊断准确性EPTB.
这种基于纳米的方法有可能发展成为EPTB可信的诊断工具包.

关键词:

MB-AuNP-I-PCR 是一个字符.在MPT-64+CFP-10中使用MPT-64.基于黄金纳米粒子的实时免疫PCR (MB-AuNP-RT-I-PCR) 的SYBR绿色磁珠合金纳米粒子.诊断诊断诊断诊断诊断诊断肺外结核是肺外结核的一种疾病.功能化黄金纳米颗粒功能化黄金纳米颗粒金纳米颗粒的金子纳米颗粒磁性珠子磁性珠子磁铁-ELISA的测试结果

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