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相关概念视频

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

254
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
254
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

840
A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
840
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

241
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
241
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

723
When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
723
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

1.1K
NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
1.1K
Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

524
The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
524

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相关实验视频

Updated: Jul 20, 2025

Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo
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基于超宽单侧带相位敏感检测的时间分辨率电子磁共振谱仪.

Shixue Zhang1, Shengqi Zhou1, Jianqing Qi1

  • 1Center of Basic Molecular Science, Department of Chemistry, Tsinghua University, Beijing 100084, China.

The Review of scientific instruments
|August 4, 2023
PubMed
概括

一种新的超宽单侧带相位敏感检测 (U-PSD) 技术增强了时间解析电子磁共振 (TREPR) 灵敏度,用于检测短寿命基. 这种方法为研究复杂的光化学系统和短暂基动力学提供了卓越的性能.

科学领域:

  • 物理化学 物理化学
  • 频谱学是一种光谱学.
  • 化学动力学 化学动力学

背景情况:

  • 短命基是许多化学和生物过程中的关键中间体.
  • 在热平衡下检测过渡激素需要高灵敏度和时间分辨率.
  • 传统的连续波电子磁共振 (EPR) 在解决快速运动事件方面存在局限性.

研究的目的:

  • 开发一种具有增强灵敏度和时间分辨率的新型时间分辨电子磁共振 (TREPR) 方法.
  • 为TREPR.引入和验证超宽单侧带相位敏感检测 (U-PSD) 技术.
  • 为了能够在很长的时间范围内检测短命基,包括处于热平衡状态的基.

主要方法:

  • 开发了一种U-PSD检测技术,与连续波EPR集成.
  • 建造了一种U-PSD TREPR光谱仪原型,采用激光闪光激发和精确的定时控制.
  • 应用U-PSD TREPR来监测短暂的基因系统,如二的激光闪光光解.

主要成果:

  • 与直接检测相比,U-PSD技术显著提高了宽带短暂信号的灵敏度.
  • U-PSD TREPR光谱仪成功检测出化学诱导的动态电子极化和热平衡EPR信号.
  • 观察到的信号跨越了从次微秒到毫秒的广泛时间尺度,证明了广泛的适用性.

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结论:

  • U-PSD技术是TREPR光谱学的可行和强大的进步.
  • 这种方法为研究复杂的光化学系统和短暂基动力学提供了卓越的性能.
  • U-PSD TREPR补充了现有的技术,在光电还原催化和人工光合作用等领域提供了更深入的机械洞察力.