在与年龄相关的干眼现象型中,甲素S抑制的效果
Jeremias G Galletti1,2, Kaitlin K Scholand1,3, Claudia M Trujillo-Vargas1,4
1Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, United States.
Investigative ophthalmology & visual science
|August 4, 2023
概括
在老年小鼠中抑制甲素S (Ctss) 改善了干眼症状,包括眼睛表面健康和角膜敏感性. 这表明,针对CTSs的疗法可能有效治疗与年龄相关的干眼疾病.
科学领域:
- 眼科医生 眼科 眼科
- 免疫学 免疫学 免疫学
- 衰老研究研究 衰老研究
背景情况:
- 年龄较大的C57BL/6J小鼠表现出高素S (Ctss) 水平.
- 老年CTSs淘汰赛小鼠表现出对与年龄相关的眼睛干燥的抵抗力.
- 甲素S在与年龄相关的干眼疾病的发病过程中发挥作用.
研究的目的:
- 为了研究 cathepsin S 抑制在缓解与年龄相关的干眼的治疗潜力.
- 评估Ctss抑制对眼睛表面参数和老年小鼠免疫细胞透的影响.
主要方法:
- 雌性C57BL/6J小鼠 (15.5-17个月大) 接受了12周的Ctss抑制剂 (RO5461111) 或标准饮食的饮食.
- 用科切-波内特审美仪评估角膜敏感度.
- 眼腺和眼部排水淋巴结通过组织学和流动细胞计量分析了免疫细胞群和炎症焦点.
- 将ctss淘汰赛小鼠与年龄相匹配的野生类型对照进行了比较.
主要成果:
- 在老年小鼠中,甲素S抑制和Ctss缺乏改善了结膜杯细胞密度和角膜机械敏感性.
- 虽然整体眼腺炎症得分没有变化,但治疗和淘汰赛小鼠的眼腺中CD4+IFN-γ+T细胞的透减少了.
- 与野生类型相比,老年CTS的淘汰眼腺显示T中心记忆细胞和改变的B细胞种群的增加 (更多的CD19+B220-和更少的CD19+B220+细胞).
结论:
- 针对cathepsin S的疗法可以有效地改善与年龄相关的干眼.
- 降低甲素S水平对眼睛表面和角膜敏感性产生积极影响.
- 需要进一步的研究,以充分阐明 cathepsin S 在衰老过程和干眼发育中的作用.
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