相关实验视频

Updated: Jul 20, 2025

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

从全转录组关联研究获得的基因作为2型糖尿病潜在的内脏脂肪组织特异性标

Haibo Tang¹, Jie Wang², Peizhi Deng²

¹Department of Metabolic and Bariatric Surgery, The Third Xiangya Hospital, Central South University, Changsha, China.

|August 4, 2023

概括

内脏肥胖导致2型糖尿病的风险增加. 研究人员确定了十个与2型糖尿病相关的内脏脂肪组织 (VAT) 特定基因,其中包括五个因果基因,提供了新的治疗点.

科学领域:

遗传学遗传学是一个
代谢疾病代谢疾病
肥胖问题研究研究

背景情况:

内脏肥胖是2型糖尿病的重要危险因素.
了解内脏脂肪组织 (VAT) 和2型糖尿病之间的遗传联系对于开发向疗法至关重要.

研究的目的:

调查内脏肥胖和2型糖尿病之间的因果关系.
在内脏脂肪组织 (VAT) 中识别导致2型糖尿病的特定基因.

主要方法:

使用双向门德尔随机化 (MR) 和多变量MR来评估因果关系.
在大型2型糖尿病GWAS数据集上进行了全转录组关联研究 (TWAS).
采用基于总结数据的MR (SMR),同定位,丰富和单细胞RNA-seq分析.

主要成果:

证实了增值税量增加和2型糖尿病之间的因果关系 (OR 2.48).
确定了十个与2型糖尿病相关的VAT特定候选基因,包括五个因果基因 (PABPC4,CCNE2,HAUS6,CWF19L1,CCDC92).
发现大多数已识别的基因可能与VAT中的脂肪细胞有关,PABPC4可能调节脂肪细胞分化和新陈代谢.

结论:

建立了新的遗传洞察力,了解增值税积累与2型糖尿病之间的关联.
突出了特定的增值税衍生基因作为2型糖尿病研究的潜在目标.
建议进行进一步的功能研究,以验证这些候选基因用于治疗开发.

关键词:

候选基因是一个候选基因.因果推理的原因推理.门德尔的随机化转录组范围的关联研究研究.2 型糖尿病 2 型糖尿病内脏脂肪组织的脂肪组织.

更多相关视频

Isolation of Viable Adipocytes and Stromal Vascular Fraction from Human Visceral Adipose Tissue Suitable for RNA Analysis and Macrophage Phenotyping

Isolation of Viable Adipocytes and Stromal Vascular Fraction from Human Visceral Adipose Tissue Suitable for RNA Analysis and Macrophage Phenotyping

Published on: October 27, 2020

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Published on: April 19, 2013

相关概念视频

Genome-wide Association Studies-GWAS

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

Diabetes Mellitus: Type 2 and Gestational

Diabetes Mellitus: Type 2 and Gestational

Type 2 diabetes, characterized by insulin resistance, arises when the insulin receptors on cells lose responsiveness to insulin, diminishing the cell's capacity to take up glucose, resulting in elevated blood glucose levels. To receive a diagnosis of Type 2 diabetes, a series of blood glucose tests are necessary to assess whether the blood glucose falls within normal parameters. If the result is out of the normal range, a patient may be diagnosed as prediabetic or diabetic, depending on the...