脱水乳抑制了Candida albicans的生长和生物膜的形成
Jingxiao Zhang1, Jian Sun1, Yu Zhang2
1Department of Emergency and Critical Care Medicine, The Second Hospital of Jilin University, 218# Ziqiang Street, Changchun, 130041, China.
AMB Express
|August 4, 2023
概括
脱水乳 (DL) 有效地抑制了Candida albicans的生长和生物膜的形成. 这种化合物通过破坏细胞膜和增加活性氧物种 (ROS) 显示出治疗菌感染的潜力.
科学领域:
- 菌类学 菌类学是指菌类学.
- 传染性疾病 传染性疾病
- 自然产品 化学 化学
背景情况:
- 虫 Candida albicans 感染对公众健康构成重大威胁.
- 有限的抗真菌药物选择需要探索新的治疗药物.
- 脱水乳 (DL) 是一种具有潜在药用功能的天然化合物.
研究的目的:
- 评估 dehydrocostus乳 (DL) 对Candida albicans的抗真菌和抗菌效果.
- 阐明DL对抗Candida albicans活动的基础机制.
主要方法:
- 使用微稀释试验来确定最小抑制度 (MIC).
- 显微镜,代谢活动测定和殖民地形成单位 (CFU) 计数评估了形态变化和粘附.
- 使用特定染色 (化,DCFH-DA) 的XTT测定和共聚焦显微镜评估了生物膜抑制,膜透性和反应性氧物种 (ROS) 生产.
- 用N-乙-氨酸进行了实验,以确认ROS的作用.
主要成果:
- DL抑制了标准的Candida物种和临床分离物的生长.
- DL减少了C. albicans的形态过渡和对表面的粘附.
- DL有效地抑制了生物膜的形成.
- DL治疗增加了细胞膜的透性和细胞内ROS的产生.
- N-乙-氨酸部分逆转了DL的抑制作用,证实了ROS的参与.
结论:
- 脱水乳 (DL) 显示出对Candida albicans的显著抗真菌和抗菌膜活性.
- DL的抗真菌机制包括诱导膜损伤和过度的ROS生产.
- DL是一个有前途的治疗候选药物,用于对抗Candida感染.
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